一株嗜酸硫氧化菌的分离(英文)  

Isolation of an Acidophilic Sulfur Oxidizing Bacterium

作　　者：程海娜[1] 胡岳华[1] 高健[1] 

机构地区：[1]中南大学资源加工与生物工程学院,中国湖南长沙410083

出　　处：《生命科学研究》2007年第2期105-109,共5页Life Science Research

基　　金：国家重大基础研究(973)计划项目(2004CB619204);国家创新研究群体科学基金项目(50621063)~~

摘　　要：从江西德兴分离得到一株嗜酸硫氧化杆菌DX-2,采用双层固体培养基进行分离纯化,对分离菌株进行了形态、生理生化特性研究及16SrRNA序列分析.该菌株为革兰氏阴性细菌,短杆状,菌体大小(0.4～0.5)μm×(1～2)μm,化能自养,可利用硫磺和硫代硫酸盐为能源生长,不能利用亚铁进行生长.以16SrRNA序列同源性为基础构建了相关种属在内的系统发育树,结果表明,DX-2与喜温硫杆菌(Acidithiobacillus caldus)处于同一进化树分支中,相似性达99%以上.考察了不同重金属离子对DX-2的生长的影响.An acidophilic sulfur-oxidizing bacterium DX-2 was isolated from Dexing area, Jiangxi province in China using enrichment medium and then purified on overlaid solid medium. The morphological, biochemical and physiological characters of DX-2 were studied. The cells of the strain DX-2 were Gram negative and rod-shaped （0.4-0.5） μm by （1-2） μm. The strain was capable of growth by using elemental sulfur or sodium thiosulfate as the sole energy sources. The strain was unable to oxidize ferrous iron. A phylogenetic tree was constructed by comparing published 16S rRNA sequences of the relative bacteria species. In the phylogenetic tree, DX-2 was the closest relative to Acidithiobacillus caldus with more than 99% sequence similarity. The effect of different heavy metals （Cu^2＋, Cr^3＋, Cd^2＋, Sn^2＋, Pb^2＋） on growth inhibition of DX-2 was investigated.

关 键 词：喜温硫杆菌 16SrRNA 分离 特性 重金属 

分 类 号：Q939.99[生物学—微生物学]