高效液相色谱法检测米雅利桑爱儿A颗粒中B族维生素  

Determination of vitamin B 6,calcium pantothenate and vitamin B 2 in Miyarisan Aiji A fine granules by HPLC

作　　者：汪洁[1] 

机构地区：[1]上海市药品检验所

出　　处：《中国药学杂志》1997年第2期106-108,共3页Chinese Pharmaceutical Journal

摘　　要：目的：测定米雅利桑爱儿Ａ颗粒中维生素Ｂ６、泛酸钙、维生素Ｂ２的含量。方法：采用高效液相色谱法，用ＷａｔｅｒｓＣＬＣＯＤＳ柱，以乙腈０．２５ｍｏｌ·Ｌ－１磷酸二氢钾（ｐＨ３．２±０．４）为流动相梯度洗脱，对乙酰氨基酚为内标，泛酸钙检测波长为２１０ｎｍ，维生素Ｂ６、内标和维生素Ｂ２的检测波长为２８０ｎｍ。结果：维生素Ｂ６、泛酸钙、维生素Ｂ２分别在２０～１３０μｇ·ｍｌ－１（ｒ＝０．９９９３，ｎ＝６），２０～１３０μｇ·ｍｌ－１（ｒ＝０．９９８６，ｎ＝６），５～３０μｇ·ｍｌ－１（ｒ＝０．９９９３，ｎ＝６）浓度范围内呈线性关系。维生素Ｂ６、泛酸钙、维生素Ｂ２平均回收率分别为９９．７％，ＲＳＤ＝０．８６％；１００．０％，ＲＳＤ＝０．９５％；９８．２％，ＲＳＤ＝１．０２％。结论：方法简便、快速、准确、重现性好。OBJECTIVE:To make an assay of vitamin B 6,calcium pantothenate and vitamin B 2 in Miyarisan Aiji A fine granules.METHOD:A HPLC method was chromatographed on Waters CLC ODS column with a mobile phase of acetonitrile 0.25 mol·L -1 potassium dihydrogen phosphose(pH 3.2±0.4)(varying from 8∶92 to 25∶75 in 25 minutes),acetaminophen as internal substance.UV detections were performod at 210 nm for calcium pantothenate and 280 nm for vitamin B 6,acetaminophen and vitamin B 2.RESULTS:The standard curves for vitamin B 6,calcium pantothenate and vitamin B 2 were linear in the range of 20～130 μg·ml -1 (r=0.999 3,n=6),20～130 μg·ml -1 (r=0.998 6,n=6),5～30 μg·ml -1 (r=0.999 3,n=6) respectively.The average recoveries of vitamin B 6,calcium pantothenate and vitamin B 2 at different concentration were 99.7%,,100.0% and 98.2% respectively,their RSD was 0.86%,0.95%,1.02%(n=5).CONCLUSION:The method is convenient,rapid,accurate and precise.

关 键 词：高效液相色谱 米雅利桑爱儿A 颗粒 维生素B6 

分 类 号：R927.2[医药卫生—药学] R977