针刺或褪黑素的中枢降压作用与延髓氨基酸递质改变的关系  

Relationship Between the Central Depressor Effect of Acupuncture or Melatonin and the Changes of Medullary Amino Acid Neurotransmitter

作　　者：王延荣[1] 王锦[1] 李莉[1] 沈霖霖[1] 曹银祥[1] 朱大年[1] 

机构地区：[1]复旦大学上海医学院生理学和病理生理学系,上海200032

出　　处：《上海针灸杂志》2007年第6期41-45,共5页Shanghai Journal of Acupuncture and Moxibustion

基　　金：国家973计划(2005CB523306);国家自然科学基金(30340071)

摘　　要：目的研究针刺降压和褪黑素(Mel)在下丘脑前核(AHA)引起应激性高血压大鼠血压降低的作用是否与延髓头端腹外侧区(rVLM)氨基酸类递质释放的改变有关,并分析Mel降压机制与针刺降压机制之间的关系。方法采用足底电击结合噪声的方法建立应激性高血压大鼠模型;针刺应用电针的方法,穴位取双侧足三里;采用中枢核团微量注射的方法给药,观察动脉血压的改变,同时应用脑内微透析的方法取样,并利用高效液相色谱-荧光检测的方法,分析透析液中氨基酸含量的改变。结果大鼠在接受应激处理后,血压升高,同时,rVLM内谷氨酸(Glu)释放量增加,应激性高血压大鼠在经针刺处理后,血压回降,同时,rVLM内Glu释放量回减。微量注射Mel至应激性高血压大鼠的AHA能使血压降低,同时,rVLM内Glu释放量也减少,而γ-氨基丁酸(GABA)和牛磺酸(Tau)释放量则增加。在rVLM微量注射GABAA受体拮抗剂荷包牡丹碱(bicuc)则可部分阻断Mel在AHA引起的降压效应。结论针刺降压与rVLM内Glu释放量减少有关,而Mel在AHA引起的降压效应则与rVLM内Glu释放量减少,以及GABA和Tau释放量增加有关。针刺降压和Mel在AHA引起的降压机制都与rVLM内Glu释放量减少有关。Objective To investigate whether changes of amino acid neurotransmitter releases in the rostral ventrolateral medulla （rVLM） were related to acupuncture and to the anti-hypertensive effect of melatonin （Mel） microinjected into the anterior hypothalamic area （AHA） of stress-induced hypertensive rats （SIHR）, as well as to compare and analyse the relationship between the both antihypertensive mechanisms of acupuncture and of Mel in the AHA. Methods Animal model of SIHR was made by electric foot shocks combined with noises. Electroacupuncture （EA） was used and ＂Zusanli＂ acupoint of both side was selected. The technique of drug microinjection into the brain was used to observe the change of blood pressure （ BP）, and synchronously, brain microdialysis was performed for collecting dialysate samples, and then the concentration of amino acid neurotransmitters in the dialysate samples was determined by high performance liquid chromatography combined with fluorescent detection （HPLC-FD）. Results After the animal re- ceived stress treatment, the BP elevated, synchronously, the release of glutamate （Glu） in the rVLM increased, and when EA was performed, the elevated BP of the SIHR decreased, simultaneously, the release of Glu in the rVLM decreased also. After Mel was microinjected of into the AHA of the SIHR, the elevated BP attenuated, meanwhile, the release of Glu decreased, and those of γ-aminobutyric acid （GABA） and taurine （Tau） increased in the rVLM. Administration of bicuculline, an antagonist of GABAA receptor, into the rVLM prior to microinjection of Mel into the AHA could partially block the depressor effect of Mel in the AHA. Conclusion The decrease in the release of Glu in the rVLM contributes to the anti-hypertensive effect of both acupuncture and Mel in the AHA in the SIHR, and the increase in the release of GABA and Tau in the rVLM is also important to Mel in the AHA.

关 键 词：针刺 血压 褪黑素 下丘脑前核 延髓头端腹外侧区 氨基酸类神经递质 

分 类 号：R2-03[医药卫生—中医学]