AAV-hTGFβ1基因体内转染羊椎间盘对髓核细胞Ⅱ型胶原的影响  

THE EFFECT OF INJECTION OF AAV-hTGFβ1 IN GOAT INTERVERTEBRAL DISC ON COLLAGEN Ⅱ

作　　者：刘勇[1] 胡鹏[1] 胡有谷[1] 齐宗华[1] 

机构地区：[1]青岛大学医学院附属医院骨科,山东青岛266003

出　　处：《青岛大学医学院学报》2007年第4期331-332,共2页Acta Academiae Medicinae Qingdao Universitatis

基　　金：国家自然科学基金资助项目(30271318)

摘　　要：目的观察腺相关病毒(AAV)介导人转化生长因子β1(hTGFβ1)基因体内转染羊椎间盘后,对其Ⅱ型胶原的影响。方法SPF级山羊8只,共取椎间盘32个,实验组、对照组各16个。实验组椎间盘显微注射AAV-hTGFβ1,对照组注射磷酸盐缓冲液(PBS)。术后4周对山羊腰椎间盘行免疫组织化学观察,采用VIDAS图像分析系统进行半定量分析。结果实验组Ⅱ型胶原表达明显高于对照组,差异有统计学意义(t=3.521,P<0.05)。结论AAV-hTGFβ1基因体内转染后能提高椎间盘细胞Ⅱ型胶原的表达。Objective To observe the effect of hTGFβ1 encoding gene mediated by adeno-associated virus （AAV） on the collagen Ⅱ of the nucleus pulposus cells of intervertebral disc of goat. Methods Thirty-two intervertebral discs from eight goats were evenly divided into two groups： group Ⅰ treated with AAV-hTGFβ1 injection and group Ⅱ with 0.01 mol/L PBS injection. Four weeks after treatment, immunohistochemistry staining and VIDAS analysis were applied to semiquantitatively analyze the expression of collagen Ⅱ. Results The expression of collagen Ⅱ in group Ⅰ was significantly stronger than that of group Ⅱ （t = 3.521, P〈0.05）. Conclusion Transfer of AAV-hTGFβ1 into the intervertebral discs could enhance the expression of collagen Ⅱ in the nucleus pulposus.

关 键 词：转化生长因子Β 胶原Ⅱ型 椎间盘 

分 类 号：R681.5[医药卫生—骨科学]