川芎嗪对小鼠急性肝损伤性脂肪肝保护作用的研究  

Protective effect of ligustrazine on acute injury fatty liver in mice

作　　者：孙玉芹[1] 高天芸[1] 周娟[1] 洪蓓蓓[1] 丁虹[1] 

机构地区：[1]武汉大学药学院药理教研室

出　　处：《中国临床药理学与治疗学》2007年第5期540-543,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:研究川芎嗪对小鼠急性肝损伤性脂肪肝的保护作用。方法:用0.5%四氯化碳花生油溶液复制小鼠急性肝损伤性脂肪肝模型。造模前1h,川芎嗪高、中、低3个剂量组分别腹腔注射川芎嗪注射液50、25、12.5mg/kg,造模后每天给药两次,连续给药7d后,测定血清中谷丙、谷草转氨酶活性,肝脏中甘油三脂、游离脂肪酸、丙二醛含量及肝脂酶和超氧化物歧化酶活性,并作肝脏HE切片。结果:与模型组比较,川芎嗪组小鼠血清中谷丙、谷草转氨酶活性均降低(P<0.05),肝脏中游离脂肪酸、甘油三酯、丙二醛含量均降低(P<0.05或P<0.01),肝脂酶和超氧化物歧化酶活性升高(P<0.05),肝脏脂肪变性明显减轻。结论:川芎嗪可改善急性肝损伤性脂肪肝中脂肪的堆积,对肝脏有较明显的保护作用。其保肝机制可能与降低甘油三酯,促进游离脂肪酸的β-氧化,抗脂质过氧化作用有关。ABSTRACT AIM： To investigate the protective effect of ligustrazine on fatty liver induced by carbon tetrachloride in mice and study the possible mechanisms. METHODS： The fatty liver model was established by 0.5% carbon tetrachloride （CCl4）-oil solution i.p. on the first day of the experiment. One hour before the model was made, mice in high-dose, middle-dose and low-dose groups were given ligustrazine 50, 25 and 12.5 mg/kg i. p., respectively. Furthermore, the model group, Tiopronin group and different dosages of ligustrazine groups were taken normal saline, Tiopronin 200 mg/kg, and ligustrazine 50, 25 and 12.5 mg/kg i.p. , respectively, twice a day for 7 days. The indexes of liver function such as levels of GOT and GPT in serum were evaluated. The liver lipid and lipid superoxidation were measured, and pathology was examined. RESULTS： Compared with the model group, the activities of ALT and AST significantly decreased in serum （ P 〈 0.05） ; Ligustrazine markedly decreased the contents of triglycerides （TG） （ P 〈 0.05） and free fatty acid （FFA） （ P 〈 0.01） in liver tissue; the activities of lipase and SOD were higher （ P 〈 0.05）, and the content of malondialdehyde （MDA） decreased markedly （ P 〈 0.05 or P 〈 0.01） in liver tissue. CONCLUSION： Ligustrazine improves the protective effect on injury liver in mice and decreases the deposition of triglycerides in liver. The possible mechanism is that ligustrazine can reduce the TG deposition, promote the β-oxidation of FFA and decrease the lipid peroxidation injury.

关 键 词：川芎嗪 脂肪肝 脂质 游离脂肪酸 

分 类 号：R965.2[医药卫生—药理学]