周围神经联合活化巨噬细胞移植修复小鼠脊髓损伤  

Repair of spinal cord injuries in mice using peripheral nerve and activited macrophages transplantation

作　　者：刘洪智[1] 周路纲[1] 贺兰英[1] 王磊[1] 孙煜杰[1] 

机构地区：[1]烟台毓璜顶医院骨科,山东264000

出　　处：《中国矫形外科杂志》2007年第13期1015-1018,共4页Orthopedic Journal of China

摘　　要：[目的]探讨周围神经联合活化巨噬细胞联合移植修复小鼠脊髓损伤(spinal cord injury,SCI)的效果。[方法]首先获取活化巨噬细胞及周围神经备用,将所有实验用小鼠均制成脊髓半切模型,最后获得符合实验要求的小鼠共81只。将其随机分为4组,A组20只,为巨噬细胞移植组;B组20只,为周围神经移植组;C组21只为周围神经与巨噬细胞联合移植组;D组20只,为空白对照组。每一组内再随机分为3组,第1组7只,用来观察肢体运动恢复情况及神经电生理变化情况;第2组7只小鼠,用来进行脊髓组织切片免疫组化检查;第3组6只小鼠(C3组7只),作为实验补充组。从术后第1周到术后第12周,通过术后BBB运动功能评分。SEP检测、免疫组化组织学观察来评估SCI的修复情况,采用SPSS12.0软件包用ONEWAYANOVA法进行数据处理,组间差异选用q检验进行两两比较,进行统计学处理。[结果]小鼠肢体运动功能评分及SEP检测结果:C1组与其他各组均存在差异(P<0.01),动物肢体功能恢复显著。A1组和B1组与其他两组存在差异(P<0.01),两组间相比,B1虽较A1组评分略高,但两者无统计学差异(P>0.05)。动物肢体功能恢复明显;免疫组化染色结果:C2组NF阳性细胞数目在SCI处的分布与其他三组有显著性差异。NF阳性细胞分布密度明显大于其他三组(P<0.05)。[结论]周围神经与活化巨噬细胞联合移植能有效促进小鼠脊髓半切损伤的修复。[Objective] To explore the effect of spinal cord injury （SCI） repair by peripheral nerve combined with activitied macrophages graft. [Method] Fisfly the preparation of the activated macrophages and fabrication of the peripheral nerve were done. 81 BALB/C mice were grouped in A, B, C, D groups randomly after their spinal cord were hemi-resected. 20 mice of group A were dealed with activated macrophages graft; 20 of group B were dealed with peripheral nerve graft; 21 of group C were transplanted both two graftes; 20 of group D were contral group. Each of the 4 groups was respectively divided into 3 subgroups randomly. 7 of subgroup 1 were investigated by their restoration of the motor function and improvement of the somatosensory evoked potential （SEP）. 7 of subgroup 2 were tabken for histological examination. The spinal cords were fixed and cut into longitudinal frozen sections, and were immuneostained and stained with hematoxylin and eosin （HE）. The numbers of cells or neurofilaments were counted under microscope. 6 of subgroup 3 （7 of C3 ） were used as complement ones. All the results were recorded from 1 to 12 weeks postoperative and analyzed statistically using the SPSS 12. 0 software package. [ Result] The BBB scale and the SEP wave： there was a significant difference between group C1 and the other three groups, there was a significant difference between group A1, B1 and D1, too （ ONE WAYANOVA, P 〈0. 01 ）. It was proved that the motor functional restoration of group A1, B1 and C1 was obvious. The number of the NF positive cells： there was a significant difference between the group C,2 and the other three groups. [ Conclusion] The peripheral nerve combined with activated m acrophages transplantation can promote the SCI repair efectively.

关 键 词：SCI 周围神经 活化巨噬细胞 SCI模型 

分 类 号：R651.2[医药卫生—外科学]