用微卫星标记研究樱桃品种间的亲缘关系(英文)  

Phylogenetic relationships among cherry genotypes using microsatellite markers

作　　者：艾呈祥[1] 辛力[1] 张力思[1] 魏海蓉[1] 苑克俊[1] 孙清荣[1] 金松南[1] 刘庆忠[1] 

机构地区：[1]山东省果树研究所,山东省果树生物技术育种重点实验室,山东泰安271000

出　　处：《果树学报》2007年第4期460-465,共6页Journal of Fruit Science

基　　金：山东省农科院青年基金项目(2005YQ013);科技部中澳国际科技合作项目(2006DFA33130);国家"863"计划资助项目(2006AA100108)

摘　　要：从来自不同地理区域的36个樱桃基因型中筛选了33对不同樱桃品种的微卫星引物,以鉴定和描述他们的基因型并确定亲缘关系。33对微卫星引物中,29对获得了预期的扩增片段,17对引物在所研究的36个基因型中产生了多态性高、稳定扩增片段,17个位点共检测到71个等位基因。微卫星分子标记技术可以准确的鉴别所有樱桃品种的基因型,为微卫星序列的通用性提供强有力的证据,可以用蔷薇科李属的序列来区分樱桃品种的不同基因型,根据不同品种的地理起源及其亲缘关系用UPGMA聚类分析法对其基因型进行分类。A collection of 36 cherry genotypes from diverse geographic areas were fingerprinted with 33 microsatellite primer pairs developed in different species of Prunus in order to identify and characterize the genotypes and establish their genetic relations. 29 of these primer pairs produced meaningful amplifications and 17 produced polymorphie repeatable amplification patterns among the 36 genotypes studied. A total of 71 alleles were detected for the 17 loci. All the genotypes studied could be unequivocally distinguished with the combinations of the mierosatellite markers used. The results provided evidence for cross-species applicability of mierosatellite sequences, which allows the discrimination of different genotypes within a fruit species with sequences developed in other species. UPGMA cluster anlaysis of the similarity data was used to group the genotypes in respect to their geographic origin and/or their pedigree information.

关 键 词：樱桃 品种特征 亲缘关系 微卫星 

分 类 号：S662.5[农业科学—果树学]