G418抗性筛选标记在板栗疫病菌中的应用  

G418-resistance as a dominant selectable marker in Cryphonectria parasitica

作　　者：蓝秀万[1] 陈敏玫[1] 桂磊[1] 范云燕[1] 甘文剑[1] 陈保善[1] 

机构地区：[1]广西大学生命科学与技术学院

出　　处：《广西农业生物科学》2007年第B06期7-11,共5页Journal of Guangxi Agricultural and Biological Science

基　　金：国家自然科学基金重点项目(30130020);国家杰出青年科学基金(39925003);教育部高校骨干教师资助计划(2000);广西自然科学基金(桂自科0229001)

摘　　要：板栗疫病菌-低毒病毒系统,是一个优良的研究植物病原真菌致病和病毒-宿主相互作用分子机制的模型。随着研究的深入,原有的苯菌灵和潮霉素两个遗传转化筛选标记已不能满足研究需要。文章报道构建一个由构巢曲霉trpC启动子驱动的G418抗性Neo基因盒,并成功应用于板栗疫病菌的遗传转化研究。Hypovirus/ Cryphonectria parasitica is an excellent experimental system to study the regulation of fungal virulence and virus-host interaction. Although considerable progress in molecular genetics of C, parasitica has been made during the past years, there is still an urgent need for efficient selectable new markers for multiple gene knockouts. In this study, we developed a new Neo expression cassette under the control of the Aspergillus nidulans trpC promoter and that serve as a dominant drug-resistance marker for C. parasitica.

关 键 词：板栗疫病菌 G418抗性 遗传转化 

分 类 号：S436.64[农业科学—农业昆虫与害虫防治]