马立克氏病毒pp38基因的克隆测序和B细胞抗原表位预测  

Cloning,sequencing and B cell epitopes prediction of pp38 gene of Marek′s disease virus

作　　者：褚秀玲[1] 苏建青[2] 靳书刚[2] 陈傲第[1] 王鲁[1] 王新[1] 郭志廷[1] 王春元[1] 伊鹏菲[1] 韦旭斌[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]河北北方学院动物科技学院,河北张家口075131

出　　处：《中国兽医学报》2007年第4期451-454,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30170698)

摘　　要：对Ⅰ型马立克氏病毒的pp38基N进行了扩增、克隆及序列测定。采用DNAStar Protean软件对pp38蛋白的二级结构、亲水性和抗原性指数等参数进行分析，并预测其B细胞表位分布。结果表明，pp38基N长873bp，包含完整的开放阅读框架，编码290个氨基酸。蛋白的B细胞表位可能位于第1～11，18～162，180-217和274-290位等氨基酸区域内或附近。本试验为pp38蛋白的表达和抗体制备奠定了理论基础。The DNA of pp38 gene of serotype Ⅰ Marek＇s disease virus was amplified and cloned,and then sequenced. Amino acid sequence of pp38 on secondary structure and hydrophilicity and antigenic index were analyzed using DNAStar Protean software,with the B cell epitopes to be predicted. The sequence analysis shows that DNA of the pp38 gene is composed of 873 bp nucleotides containing whole open reading frame of pp38 gene and coding 290 amino acids. B cell epitopes of pp38 located in or adjacent to amino acids 1-11,18-162,180-217 and 274-290. The results provid the theoretical references for preparing the antibodies against pp38 and its expressing.

关 键 词：马立克氏病毒 pp38蛋白 测序 B细胞表位 

分 类 号：S852.65[农业科学—基础兽医学]