腹水综合征对肉鸡肝脏、心肌和肠黏膜线粒体抗氧化能力的影响  

The influence on the antioxidative capacity of mitochondria in liver and heart in broiler chickens with pulmonary hypertension

作　　者：臧莹安[1] 丁发源[2] 向瑞平[3] 唐兆新[3] 王小龙[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]广东省农业科学院畜牧研究所,广东广州510640 [3]华南农业大学,广东广州510642

出　　处：《中国兽医学报》2007年第4期558-561,共4页Chinese Journal of Veterinary Science

基　　金：中国博士后科学基金资助项目(2003033411)

摘　　要：采取分组对比的方法(对照组,肉鸡腹水征发病组,治疗组)分别检测肝组织、肠黏膜、心肌线粒体中的MDA含量,SOD、GSH-PX的活性。结果显示,发生腹水综合征的肉鸡,其肝脏、心肌和肠黏膜线粒体MDA活力呈现先显著降低(1、2周时)、后显著提高(3、4、5周时)的变化趋势(P<0.05)而与发病组线粒体MDA活力先降后升的动态变化趋势相反,治疗组线粒体MDA的活力则呈现先升后降的趋势,最终显著阻止了自由基的产生。线粒体SOD和GSH-PX活力的变化趋势则与MDA相反。提示:肉鸡腹水综合征显著诱导了肉鸡组织细胞和线粒体的脂质过氧化作用,显著降低了肉鸡组织细胞和线粒体的抗氧化能力;药物能有效地改善这一过程。A total of 120 broilers were divided randomly into three groups that were respectively treated as healthy group, the pulmonary hypertension syndrome （PHS） group and the vitamin treatment group. The liver, heart and mucous membrane of intestine, were taken from 6 killed birds per group, the mitochondria were determined for the evaluations of the concentrations of malondialdehyde （MDA）, superoxide dismutase （SOD）, glutathione peroxidase （GSH-PX）. The results indicated that the MDA level in PHS group decreased significantly in the first 2 weeks （P 〈 0.05）, and increased significantly during the following 3 weeks （P 〈 0.05）, and the activities of T-SOD and GSH-PX is opposite. After the treatment of Vitamin C,all these guideline is changed following opposite way. It might be concluded that the pulmonary hypertension syndrome （PHS） in broiler chickens induced markedly the lipid peroxidation of mitochondria and cells, and increased clearly the free radicals, and reduced significantly the antioxidative capacity; the Vitamin C significantly stopped lipid peroxidation induced by PHS, and eliminated markedly productions of a lot of free radicals, and increased significantly the antioxidative capacity.

关 键 词：肉鸡 腹水综合征 线粒体 自由基 MDA SOD GSH—Px 

分 类 号：S858.31[农业科学—临床兽医学]