山羊肌肉生长抑制素(MSTN)基因HphI酶切多态性分析  

Polymorphism analysis of the MSTN gene digested with HphⅠ of goat

作　　者：刘铮铸[1] 李祥龙[1] 巩元芳[1] 李金泉[2] 冯敏山[1] 

机构地区：[1]河北科技师范学院动物科学系,河北昌黎066600 [2]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018

出　　处：《中国兽医学报》2007年第4期591-594,共4页Chinese Journal of Veterinary Science

基　　金：河北省自然科学基金资助项目(C2005000241);河北省教育厅资助项目(B2004513)

摘　　要：采用限制性内切核酸酶Hph对山羊肌肉生长抑制素(MSTN)基因的379bp扩增产物进行了PCR-RFLP多态性分析,检测到3种基因型,其酶切位点分别由2种共显性基因控制;对该片段的纯合型分别克隆、测序发现,BB型在第1661位由T→C。上述结果为首次试验证实山羊MSTN内含子2(379bp)区域存在多态性。Myostatin （MSTN） gene was amplified and an uniform fragment of 379 bp was obtained in goat. The 379 bp PCR product was digested with restriction endomuclease Hph Ⅰ and its genetic polymorphism was investigated by PCR-RFLP. Three kinds of genotypes were found in these populations,which were controlled by two alleles. The amplified fragments of AA and BB of the fragment were cloned and sequenced. The result showed that in 379 bp BB genotype had one substitution mutation at 1 661 site（T→C）. The result above-mentioned first confirmed that there are polymorphisms in intron 2（379 bp） region of MSTN gene.

关 键 词：山羊 肌肉生长抑制素 遗传多态性 RFLP Hph Ⅰ 

分 类 号：S858.27[农业科学—临床兽医学]