炙甘草汤及其有效成分配伍对哇巴因所致心肌触发活动的影响  

Effects of ZhiGanCao Decoction,Its Effective Ingredients and Their Combinations on Triggered Activity of Isolated Myocardium Induced by Ouabain and High Ca2+

作　　者：周丽娟 

机构地区：[1]厦门金日制药有限公司,厦门361100

出　　处：《中国天然药物》2007年第4期301-305,共5页

基　　金：国家自然科学基金资助项目(No39860084)~~

摘　　要：目的:探索炙甘草汤及其有效成分配伍对哇巴因所致触发活动的影响及作用机理。方法:应用微电极技术及单相动作电位技术观察炙甘草汤及其有效成分甘草酸、人参总皂苷、麦冬总皂苷和配伍对哇巴因所致触发性离体心肌触发活动的影响。结果:(1)哇巴因(0.5×10-6mmol·mL-1)高钙台氏液灌流可引起豚鼠离体左心室乳头肌触发活动。炙甘草汤、甘草酸单铵盐、人参总皂苷、麦冬总皂苷均可抑制高钙-哇巴因所致触发活动,缩短触发活动时程,降低触发活动幅度,减少触发活动发生率;当甘草酸单铵盐、人参总皂苷、麦冬总皂苷配伍时,也可抑制触发活动的发生,且3种成分配伍作用优于两两配伍,两两配伍的作用又优于单个有效成分。结论:(1)炙甘草汤、甘草酸单铵盐、人参总皂苷、麦冬总皂苷均有抗触发活动的作用;(2)甘草酸单铵盐、人参总皂苷、麦冬总皂苷为炙甘草汤的有效成分,3种成分配伍时对抗触发活动有协同作用。AIM： To study the effects and the mechanism of ZhiGanCao Decoction （ZGCD）, its effective ingredients and their combinations on the triggered activity（TA） induced by ouabain and high Ca^2＋ . METHODS：Using the standard intracellular microelectrode techniques,the effects of ZGCD, its effective ingredients and their combinations were observed on the ouabain-induced TA in guinea pig myocardium isolated. RESULT： Ouabain （0.5×10^-6mmol·mL^-1 ） and high calcium solution can produce TA in the isolated guinea pig myocardium obviously, while ZGCD, MG, GTS, OTS and the combinations can inhibit TA induced by ouabain（0.5×10^-6mmol·mL^-1 ） and high calcium solution, shorten the duration of TA（TA-A）, cut down the ample of TA（TA-A） and decrease the incidence of TA（TA-I）. The effects of three effective ingredients＇ combination were better than those of any two effective ingredients＇ combination, the effects of any two effective ingredients were better than those any effective ingredient. CONCLUSION： （ 1 ） ZGCD, MG, GTS and ors can inhibit TA; （2） MG, GTS and OST are the effective ingredients in ZGCD, the combinations of them show synergism.

关 键 词：炙甘草汤 有效成分 配伍 触发活动 

分 类 号：R285.5[医药卫生—中药学]