壳寡糖抑制肿瘤作用的实验研究  

The study on inhabitory effects of chitosan oligosaccharide on tumor growth in vivo and in vitro

作　　者：官杰[1] 罗晓庆[1] 王琪[1] 李殿俊[1] 

机构地区：[1]齐齐哈尔医学院免疫教研室,齐齐哈尔161042

出　　处：《中国免疫学杂志》2007年第5期421-425,共5页Chinese Journal of Immunology

基　　金：齐齐哈尔医学院自然科学基金资助项目(项目编号:XY2004-44-21)

摘　　要：目的:通过建立H22肝癌荷瘤鼠作为实验动物模型,研究壳寡糖对肿瘤抑制作用的影响。方法:选择小鼠体内注射的方法,将不同浓度壳寡糖注入荷瘤小鼠体内,观察肿瘤生长情况,对其进行免疫功能的测定;同时通过MTT比色法观察壳寡糖对LH-7人肺癌细胞的体外生长抑制作用。结果:壳寡糖对肿瘤的生长有抑制作用;壳寡糖可提高荷瘤小鼠血清的IL-2和IFN-γ含量,增加荷瘤小鼠免疫器官脾脏和胸腺重量;显微镜观察肿瘤组织出现坏死;壳寡糖对LH-7细胞生长有抑制作用,抑瘤率与浓度有关,与时间无关;透射电镜显示细胞有凋亡趋势。结论:壳寡糖可抑制肿瘤生长,提高机体免疫功能;同时壳寡糖可能诱导LH-7肿瘤细胞凋亡。Objective：To investigate the inhibitory effects of chitosan oligosaccharide on tumor growth in vivo. Methods：Murine tumor cell line H22 was inoculated into. Then different doses of chitosan oligosaccharide were injected into the mice-bearing H22 liver carcinoma, IL-2 and IFN- γ in the sera were measured by ELISA assay. The in vitro anti-proliferation activity of chitosan oligosaccharide on the lung carcinoma LH-7 cells was evaluated by MTT assay. Results：Chitosan oligosaccharide inhibited in vivo the growth of H22 tumor cells, with increasing the content of IL-2 and IFN-γ in sera of the tumor-bearing mice. The weight of spleen and thymus of the mice were increased when compared with those of control group ; tissue necrosis was observed in the tumor in situ ; chitosan oligosaccharide also inhibited the growth of LH-7 cells in vitro. The inhibitory effect was shown in a concentration dependent pattern, but not correrlated with incubation time. The early characteristics of apoptosis of LH-7 cell could be observed under the transmission electronic microscopy. Conclusion： Chitosan oligosaccharide inhibits proliferation of tumor and improves immunity function of the hosts, and might induce apoptosis of LH-7 cells.

关 键 词：壳寡糖 肿瘤 抑制 细胞培养 

分 类 号：R931.74[医药卫生—生药学]