恶性肿瘤患者CD4^+CD25^+/CD4^+CD25^(high)调节性T细胞的研究  

CD4^+CD25^+/CD4^+CD25^(high) regulatory cells in peripheral blood of cancer patients

作　　者：姚军霞[1] 刘莉[2] 黄士昂[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院干细胞中心,武汉430022 [2]华中科技大学协和医院肿瘤中心,武汉430022

出　　处：《中国免疫学杂志》2007年第5期430-433,437,共5页Chinese Journal of Immunology

基　　金：国家杰出青年基金资助项目(No30225038)

摘　　要：目的:探讨恶性肿瘤患者外周血CD4+CD25+/CD4+CD25high调节性T细胞(Regulatory T cell,Treg)水平的特点及其临床意义。方法:采用流式细胞术检测Treg水平,并进行分层分析。结果:62例恶性肿瘤患者外周血中CD4+CD25+Treg、CD4+CD25high Treg占T细胞的百分比分别为19.61%±8.17%和4.20%±1.90%,高于正常对照组(分别为P<0.05和P<0.001),它们与NK细胞呈负相关(r分别为-0.2361和-0.306)。随疾病进展,CD4+ CD25+Treg水平升高,肿瘤进展期(IV期)与前3期比较有极显著差异(P<0.001)。CD4+CD25high Treg占CD4+T细胞的百分比率逐渐升高,中期(Ⅲ期)患者与早期患者、正常对照组比较有极显著差异(P<0.001);晚期患者与中期患者比较有极显著差异(P<0.001)。结论:恶性肿瘤患者外周血CD4+CD25+/CD4+CD25high Treg水平的升高,与恶性肿瘤免疫功能低下及肿瘤的发生发展密切相关。Objective：To investigate the frequencies of CD4^＋ regulatory T（Treg） cells（CD4^＋CD25^＋/CD4^＋CD25^high） in peripheral blood of cancer patients, providing the opportunity to determine whether cancer patients exhibit an expanded CD4^＋ CD25^high pool. Methods：The frequency of CD4^＋CD25^＋/CD4^＋CD25^high Treg in the peripheral blood of 62 cancer patients and 15 controls was determined by flow cytometry. Results： Compared with those of healthy control, the frequency of CD4^＋CD25^＋/CD4^＋CD25^high in the peripheral blood of cancer patients showed a significant increase[ （ 19. 61 ±8. 17）%/（4. 20 ± 1.90）% ,P 〈0. 05 ,P 〈0. 001, respectively]. There was negative relation between the level of CD4^＋CD25^＋/CD4^＋CD25^high and that of NK（ r = - 0, 236 1, - 0. 306, respectively）. Compared with those in patients of stage（ Ⅰ ＋Ⅱ ） and stage Ⅲ, the frequency of CD4^＋ CD25^＋ in patients of stage IV showed significant increase（P 〈0. 001 ）. Compared with the level in healthy controls and patients of stage（ Ⅰ ＋ Ⅱ ）, the frequency of CD4^＋ CD25^highT cells in patients of advanced stage [ Ⅲ ： （ 3.52 ±1.51 ） %, n = 21 ; Ⅳ ： （4. 85 ± 1.67 ） %, n = 26 ] was significantly higher（P 〈 0. 001, both）, and there was significant difference between the frequency of CD4^＋CD25^＋/CD4^＋CD25^high in patients of stage Ⅲ and that of stage Ⅳ. Conclusion： The relative increasement in CD4^＋CD25^＋/CD4^＋CD25^high regulatory T cells may be related to immunosupression and tumor progression in patients with malignancies.

关 键 词：恶性肿瘤 CD4^+CD25^+/CD4^+CD25^high调节T细胞 流式细胞仪 

分 类 号：R392.12[医药卫生—免疫学]