苯甲酸雌二醇对BALB/c小鼠系统性红斑狼疮模型诱导的影响  

Influence of estradiol benzoate on induction of the model of systemic lupus erythematosus in BALB/c mouse

作　　者：唐小云[1] 吕昌龙[1] 张晓莉[2] 李霞[2] 

机构地区：[1]中国医科大学基础医学院免疫教研室,沈阳110001 [2]牡丹江医学院,牡丹江157011

出　　处：《中国免疫学杂志》2007年第5期452-455,共4页Chinese Journal of Immunology

摘　　要：目的:探讨不同剂量的苯甲酸雌二醇(Estradiol benzoate)对BALB/c小鼠系统性红斑狼疮(Systemic lupusery-thematosus,SLE)模型诱导的影响。方法:BALB/c小鼠卵巢摘除后用同系小鼠ConA活化的脾细胞(5×107)免疫3次,同时肌注不同剂量苯甲酸雌二醇,6周后以ELISA法检测血清抗核抗体(ANA)、抗组蛋白抗体(AHA)及血清和肾组织匀浆雌二醇(Estradiol,E2)水平,H.E、PAS染色观察肾脏病理改变,透射电镜检查肾脏超微结构的改变,直接荧光法检查IgG类免疫复合物的沉积。结果:经同系小鼠ConA活化的脾细胞免疫的BALB/c小鼠均发生红斑狼疮样改变,但不同剂量苯甲酸雌二醇组小鼠的自身抗体的产生及肾脏病理损害差异显著(P<0.05)。结论:机体不同的雌二醇水平与SLE的病变程度相关。Objective：To explore the influence of estradiol benzoate in induction of BALB/c mouse systemic lupus erythematosus model. Methods： Gonadectomied BALB/c mice were immunized with homologous spleen cell activated by concanavalin A three times, and at the same time injected with different doses estradial benzoate. Serum antinuclear antibody, antihistone antibody and estradiol in serum and kidney tissue homogenate were detected by enzyme linked immunosorbent assay. Kidney lesions were detected by hematoxylin-eosine（HE） and periodic acid-Schiff（PAS） staining, and by transmission electron microscope for detecting electron-dense agent as well as by direct fluorometric method for detecting immunoglobulin G antigen-antibody complex. Results. BALB/c mice immunized with homologous spleen cells activated by concanavalin A all developed systemic lupus erythematosus, but a statistically significant variation was observed in both autoantibody production and kidney damage were observed with a statistically significant variatron in the groups receiving different dosage of estradiol benzoate group（ P 〈 0. 05 ）. Conclusion：Different level of oestrogen influences the development of systemic lupus erythematosus.

关 键 词：雌二醇 系统性红斑狼疮 抗核抗体 抗组蛋白抗体 狼疮性肾炎 

分 类 号：R593.24[医药卫生—内科学]