ONO-AE-248诱导中性粒细胞非凋亡性程序化死亡线粒体形态结构的变化  

The structural alterations of mitochondria in ONO-AE-248-induced non-apoptotic programmed cell death of neutrophils

作　　者：羊建[1] 刘佳佳[1] 薛晓婕[1] 张姿[1] 何浩[1] 

机构地区：[1]泸州医学院分子生物学实验室,泸州646000

出　　处：《中国免疫学杂志》2007年第6期492-494,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(30371305)

摘　　要：目的:探讨线粒体形态结构的变化在ONO-AE-248诱导的中性粒细胞非凋亡性程序化死亡中的作用及意义。方法:Ficoll法分离健康志愿者外周静脉血中性粒细胞,与ONO-AE-248共同培养,透射电子显微镜观察线粒体形态结构的改变;流式细胞术检测线粒体膜势能的变化。结果:ONO-AE-248刺激6小时,中性粒细胞线粒体显著肿胀,嵴断裂,数目增多;ONO-AE-248早期迅速导致中性粒细胞线粒体膜势能的溃散。结论:线粒体变化是ONO-AE-248诱导中性粒细胞非凋亡性程序化死亡区别于凋亡的早期形态学特征事件之一。线粒体可能在ONO-AE-248诱导的中性粒细胞非凋亡性程序化细胞死亡中扮演更为关键性的角色。Objective ：To investigate the structural alterations of mitochondria and its role in neutrophil death induced by ONO- AE-248. Methods：Human neutrophils were cultured in vitro with ONO-AE-248 （5 × 10^-5 mol/L）and medium alone. Transmission electron microscopy（TEM） was used to detect the structural alterations of mitochondria and the level of mitochondria membrane potential by flow cytometry using mitocapture dying. Results： ONO-AE-248 resulted in extremely swollen mitochondria within 6 hours. Meanwhile, a rapid loss of mitochondrial membrane potential of neutrophils occurred, especially in 3 hours and 6 hours. There were obviously differences between spontaneous apoptosis and non-apoptosis programmed cell death induced by ONO-AE-248. Conclusion： The experiment results suggest that changes of mitochondrial structure and function be typically morphological, physiological and biochemical features in this unique form of neutrophil death, and that the mitochondrial pathway might play a more important role in ONO-AE-248- induced death of neutrophils.

关 键 词：线粒体 中性粒细胞 ONO-AE-248 凋亡 非凋亡性程序化细胞死亡 

分 类 号：R392.12[医药卫生—免疫学]