碳糊电极阳极吸附伏安法测定诺氟沙星  

Anodic Adsorptive Voltammetric Determination of Norfloxacin Using Carbon Paste Electrode

作　　者：易兰花[1] 王俊芬[2] 黎拒难[1] 高朋[1] 

机构地区：[1]湘潭大学化学学院,湘潭411105 [2]湘潭大学图书馆,湘潭411105

出　　处：《理化检验（化学分册）》2007年第1期57-59,62,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：中科院长春应用化学研究所电分析化学国家重点实验室基金项目(No.SKLEAC200510)

摘　　要：报道了用碳糊电极阳极吸附伏安法测定诺氟沙星的方法。在0.40 mol.L-1的NH4OAc-HOAc（pH 4.51）缓冲溶液中,诺氟沙星在碳糊电极（CPE）上有一灵敏的吸附氧化峰,峰电位为1.16 V（vs.SCE）。该氧化峰的二阶导数峰电流与诺氟沙星的浓度在4.0×10^-9~4.0×10^-7mol·L^-1（富集90 s）范围内呈线性关系,相关系数为0.996,检出限为1.0×10^-9mol·L^-1（S/N=3,富集110 s）;应用于人体尿液中诺氟沙星含量的测定,方法的回收率为99.1%~104.0%。文中探讨了诺氟沙星在碳糊电极上的伏安性质和电极反应机理。A highly selective and rapid anodic adsorptive voltammetric method was developed for the determination of trace amounts of Norfloxacin by using a carbon paste electrode （CPE）. The Norfloxacin was adsorbed on the surface of the CPE in a medium of 0. 40 mol · L^-1 NH4OAe-HOAc buffer （pH 4.51） yielding an oxidation peak at 1.16 V （vs. SCE）. The linear relationship between the peak current and Norfloxacin concentration was found in the range of 4.0× 10^-9~4. 0× 10^-7mol · L^-1 with correlation coefficient of 0. 996. The detection limit was found to be 1.0×10^-9mol · L^-1 （S/N=3） with acummulation for 110 s. Mechanism of the electrode reaction was discussed. The proposed method has been applied to the direct determination of trace amounts of Norfloxacin in urine samples with RSD＇s less than 4%, and recoveries in the range of 99.1%-104.0%.

关 键 词：诺氟沙星 阳极吸附伏安法 碳糊电极 

分 类 号：O657.31[理学—分析化学]