不同胁迫预处理提高水稻幼苗抗寒性期间膜保护系统的变化比较  

COMPARISON OF THE CHANGES OF MEMBRANE PROTECTIVE SYSTEM IN RICE SEEDLINGS DURING ENHANCEMENT OF CHILLING RESISTANCE BY DIFFERENT STRESS PRETREATMENT

作　　者：曾韶西[1] 王以柔[1] 李美如[1] 

机构地区：[1]中国科学院华南植物研究所,广州510650

出　　处：《Acta Botanica Sinica》1997年第4期308-314,共7页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金

摘　　要：通过比较盐、冷和热激三种不同胁迫预处理提高水稻(Oryza sativa L.)抗寒性过程中膜保护系统的变化,探讨植物交叉适应机理。结果表明,水稻幼苗经不同胁迫预处理均可提高幼苗的抗寒性。与未预处理苗相比,不同胁迫(冷、热、盐)预处理之间在处理后、低温伤害后及恢复2d后的3个不同时期膜酶促和非酶促保护系统及同工酶酶谱变化各有异同,既有部分共性,也有其独特性。The changes of the membrane protective system in rice (Oryza sativa L.) seedlings during the enhancement of chilling resistance with salt, cold and heat shock pretreatment were compared to explore the mechanism of cross adaptation of the plants. The different stress pretreatments maintained the cell membrane stability and enhanced the chilling resistance of the seedlings at the chilling stress. By comparison with nonpretreated seedlings, there were similarities and specificities among the three stress pretreated seedlings on the changes of enzymic and nonenzymic protective system as well as isoenzymes zymograms. The SOD activities in all the three pretreated seedlings were very similarly higher than those of the nonpretreated seedlings at any tested time. The catalase (CAT) activities of heat shock pretreated seedlings were always higher than those of the nonpretreated seedlings at the three stages. In contrast, the CAT activities of salt pretreated seedlings were lower. Those of the cold pretreated seedlings were lower after the pretreatment and chillings injury, however were higher after the rescovery for 2 days. The peroxidase (POD) activities of the salt and cold pretreated seedlings were lower or identical with those of the nonpretreated seedlings after the pretreatment and lower than those of the nonpretreated seedlings after chilling injury; those of the heat shock pretreated seedlings were egual to the nonpretreated seedlings. However, the activities in all the three pretreated seedlings were higher after the rescovery for 2 days. The contents of ascorbic acid (AsA) and the reduced form of glutathione (GSH) in cold pretreated seedlings were markedly higher than those of nonpretreated seedlings at the three tested time. In salt and heat shock pretreat-ed seedlings, the contents of AsA and GSH were lower or slightly higher after the treatment and chilling injury, but were higher after the rescovery for 2 days. Using polyacrylamide gel elec-trophoresis analysis, the zymogram of SOD isoenzymes had similar fou

关 键 词：水稻 交叉适应 胁迫 膜保护系统 抗寒性 

分 类 号：S511.01[农业科学—作物学]