黄瓜幼苗热胁迫响应基因的分离  

Isolation of heat response-related gene in cucumber seedlings

作　　者：杨寅桂[1] 娄群峰[1] 庄勇[1] 张万萍[1] 陈劲枫[1] 

机构地区：[1]南京农业大学园艺学院作物遗传与种质创新国家重点实验室

出　　处：《生态学杂志》2007年第7期1034-1037,共4页Chinese Journal of Ecology

基　　金：国家自然科学基金(30470120)

摘　　要：为了理解黄瓜耐热性或热伤害产生的分子机理,以耐热品种‘西双版纳黄瓜’和热敏品种‘二早子’为供试材料,对幼苗进行38℃热激处理2h。采用cDNA-AFLP技术比较热激前后基因表达差异,分离黄瓜热激响应基因,并利用RT-PCR技术检测分离基因的表达。结果表明:从‘二早子’中分离到chrr182(cucumber heat response related)特异片段,经比对发现该片段仅与转录修复偶联因子(transcription repair coupling factor-TRCF)同源;该基因的表达在38℃下被诱导增强;黄瓜幼苗耐热性或热伤害的发生可能与TRCF基因的响应有关。To understand the molecular mechanism of cucumber＇ s heat tolerance or heat injury, heat-tolerant cuhivar ‘Xishuangbanna’ and heat-sentitive cuhicar‘ Erzaozi＇ were selected as test materials, and their seedlings were heat treated （38 ℃ ） for 2 hours： The gene was isolated with cDNA-AFLP technique, and the gene expression was detected by RT-PCR technique. A specific fragment of heat response, chrr182 （cucumber heat response-related ）, was isolated from the cDNA of ‘ Erzaozi＇ under heat stress, and the results of blastx in GenBank of NCBI indicated that chrr182 was in homology with transcription-repair coupling factor gene. The expression of chrr182 was enhanced under the inducement at 38 ℃ for 2 hour. The heat tolerance or heat injure of cucumber seedlings could be related to the response of transcription-repair coupling factor gene under heat stress.

关 键 词：黄瓜 热胁迫 CDNA-AFLP 转录修复偶联因子 

分 类 号：S642.2[农业科学—蔬菜学]