普通小麦与鸭茅状摩擦禾的远缘杂交 Ⅲ受精和早期胚胎的发育  

Wide Hybridization Between Triticum aestivum and Tripsacum dactyloides IIIFertilization and Early Embryo Development

作　　者：邱纪文[1] 李大玮[1] 欧阳平[1] 姚庆筱[1] 

机构地区：[1]中国科学院遗传所植物细胞和染色体工程国家重点实验室

出　　处：《Acta Genetica Sinica》1997年第2期160-164,共5页

基　　金：中国科学院重大项目;中国科学院遗传研究所所长基金

摘　　要：用石蜡制片法和整体解剖法，对小麦品种Ｆｕｋｕｈｏ经鸭茅状摩擦禾花粉授粉后不同时间固定的子房样品进行了细胞胚胎学观察。结果表明，观察的１６８个授粉后１２０小时（５天）以内的小麦子房中有３２１％、０６％和２６２％分别发生了卵细胞单受精、极核单受精和卵细胞、极核双受精现象。发生卵细胞受精、极核受精和总受精频率分别为５８３％、２６８％和５８９％。对１１６个授粉后９天的小麦子房进行整体解剖，检测到有５００％的子房有幼胚。报道了小麦与鸭茅状摩擦禾杂交的受精过程和早期胚胎发育的情况。为小麦与鸭茅状摩擦禾杂交提供了高频率受精和胚形成的细胞胚胎学证据。sing wheat variety Fukuho pollinated with Tripsacum dactyloides,fertilization and early embryo development were studiedOut of 168 ovaries fixed 2～120 hours after pollination,44(262%)showed both embryo and endosperm,54(321%)showed only embryo without endosperm and 1(06%)showed endosperm without embryoThe frequencies of the egg fertilization,pole nuclei fertilization and total fertilization were 583%,268% and 589%,respectivelly58 young embryos(approximatlly 50%)were observed from 116 ovaries 9 days after pollination,indicating that the percentages of fertilization and embryo formation were significantly highCytoembryological evidence was given to demonstrate the high freqeuncies of fertilization and embryo formation in crosses between wheat and Tdactyloides

关 键 词：小麦 鸭茅状摩擦禾 远缘杂交 受精 胚胎发育 

分 类 号：S512.103.5[农业科学—作物学]