低温预处理过程中大麦花药内源激素的变化  

The Variation of Endogenous Hormones of Barley Anther in the Process of Low Temperature Pretreatment

作　　者：徐武[1] 李鸣[1] 张敬[1] 方红曼[1] 李安生[1] 

机构地区：[1]中国科学院遗传研究所

出　　处：《Acta Genetica Sinica》1997年第2期165-169,共5页

摘　　要：很多文章曾报道低温预处理可以明显提高大麦花药反应频率。但对于低温预处理的机理，至今研究报道甚少。我们应用ＥＬＩＳＡ方法测定了大麦花药低温预处理过程中内源激素ＩＡＡ、ｉＰＡ和ＡＢＡ含量。根据实验结果，推测低温预处理改变了花药内源ＩＡＡ和ｉＰＡ含量，阻断了花粉原来的发育方向，使其由配子体的发育途径转向孢子体的发育途径。t has been reported that low temperature pretreatment can greatly improve barley anther response frequencyBut little information is available to account for the mechanism of low temperature pretreatmentWe assayed the content of endogenous hormones IAA,iPA and ABA of barley anther in the process of the pretreatment using ELISA (Enzyme Linked Immunaborbent Assay)methodOn the basis of results of our test,we suggest that low temperature pretreatment changes content of endogenous IAA and iPA of anther,thus interrupts previous developmental direction of pollen and induces microspores develop from a gametophytic way to a sporophytic way

关 键 词：大麦 花药 内源激素 低温预处理 

分 类 号：S512.301[农业科学—作物学]