嗜热脂肪芽孢杆菌中转座因子IS5376的转座行为研究  

Study on Transposition Behavior of IS5376 in Bacillus stearothermophilus

作　　者：许可[1] 毛裕民[1] 沈仁权[1] 盛祖嘉[1] 

机构地区：[1]复旦大学遗传学研究所

出　　处：《Acta Genetica Sinica》1997年第2期178-182,共5页

基　　金：国家自然科学基金

摘　　要：ＩＳ５３７６和ＩＳ５３７７是在嗜热脂肪芽孢杆菌（Ｂａｃｉｌｕｓｓｔｅａｒｏｔｈｅｒｍｏｐｈｉｌｕｓ）中发现的两个转座因子。随机取样分析的结果说明，ＩＳ５３７６由ＣＵ２１染色体向质粒ｐＦＤＣ５和ｐＦＤＣ１２的转座受温度的影响，而ＩＳ５３７７则不。温度影响的原因还不清楚，从现有证据看来，这由ＩＳ５３７６本身的性质所决定。另外，测得ＩＳ５３７６的转座作用有一定程度的专一性，还测得转座后所造成的目标序列的顺向重复为４或５ｂｐ。S5376 and IS5377 are two transposable elements discovered in Bacillus stearothermophilus.Analysis of random samples revealed that the frequency of transposition of IS5376 from CU21 chromosome to plasmids pFDC5 and pFDC12 was much higher at 65℃ than that at 48℃ while that of IS5377 was very low at both 48℃ and 65℃.The exact nature of the temperature effect is obscure at present;from evidences obtained so far it is concluded that this is a consequence of the innate property of IS5376Furthermore,it was found that a certain degree of site specificity in transposition was evident and that a direct repeat of 4 or 5 bp of the target DNA appeared at the site of transposition

关 键 词：转座因子 芽孢杆菌 嗜热脂肪杆菌 

分 类 号：Q939.103[生物学—微生物学]