慢性阻塞性肺疾病模型大鼠的学习记忆改变及咖啡因的干预作用  

Learning memory deficit in model rats of chronic obstructive pulmonary disease and intervention by caffeine

作　　者：胡丽燕[1] 王小同[1] 寇雪莲[2] 吴志鹏[1] 龚永生[2] 范小芳[2] 胡良冈[1] 

机构地区：[1]温州医学院实验神经生物学研究所,温州325027 [2]温州医学院附属第二医院神经内科

出　　处：《中国行为医学科学》2007年第7期580-582,共3页Chinese Journal of Behavioral Medical Science

基　　金：浙江省自然科学基金项目（Y204453）;温州市科技局科研项目（Y2005A001）

摘　　要：目的观察慢性阻塞性肺疾病（COPD）大鼠模型学习记忆的改变，以及不同剂量咖啡因的干预作用。方法通过八臂迷宫训练筛选的SD大鼠，间歇放置于低O2高CO2密闭氧舱4周。咖啡因分两种剂量（30mg／kg和10mg／kg）口服给药。学习记忆的行为学检测先后应用八臂迷宫和Morns水迷宫实验。结果COPD大鼠模型组空间学习记忆能力下降。在八臂迷宫实验中，与对照组相比，COPD模型组的工作记忆错误（WME）为（1．00±1．41）次，增加了50％，参照记忆错误（RME）为（2．83±1．53）次增加了41．5％。咖啡因30mg／kg和10mg／kg干预组WME次数与COPD模型组相比各减少了42％和58％；但咖啡因干预并不能明显减少COPD大鼠的RME。在Morris水迷宫实验中，COPD组训练后3d的总逃避潜伏期都较对照组延长，第5天最明显，延长达32．6％；咖啡因干预组与COPD模型组相比逃避潜伏期均缩短，且第5天明显，其中10mg/kg组缩短了18．7％。结论COPD大鼠空间学习记忆受损，以短时空间记忆受损明显；咖啡因干预可以改善COPD模型的短时空间记忆，慢性咖啡因干预的量效关系不明显。Objective To investigate learning-memory deficit in the model rats of obstructive pulmonary disease（COPD） and the protective effects of caffeine on learning-memory ability. Methods The Sprague Dawley rats which had passed eight-arm maze task were interruptedly placed in a special closed cabin which was hypoxia and hypso-carbon dioxide for 4 weeks. Caffeine of two doses （30 mg/kg and 10 mg/kg） was resolved into drinking water. Ethology tests were evaluated by eight-arm maze task and Morris water maze task ordinally. Results The COPD model rats demonstrated learning-memory deficit. At eight-arm maze task, working memory errors （WME） of COPD group （ 1.00 ± 1.41 ） increased 50% than control group, and reference memory errors （RME） （ 2.83 ± 1.53 ） increased 41.5 %. Compared with COPD group, WME （0.58 ± 0.67,0.42 ± 0.51 ） of caffeine intervention group （30mg/kg and 10mg/kg） decreased 42% and 58% respectively. RME also reduced, but the reduction had no statistical significance. At Morris water maze task, total swimming time of the COPD group had prolonged during the post three days compared with control group, and it had prolonged 32.6% at the fifth day. Caffeine could shorten total swimmimg time of COPD rats. The 10mg/kg group shortened 18.7% at the fifth day. Conclusion COPD model rats have spatial learning-memory deficits and especially remarkable loss of short-term spatial memory. Caffeine intervention can improve the short-term spatial memory of COPD animal model. There is no obvious doseeffect relationship in chronic caffeine treatment.

关 键 词：慢性阻塞性肺疾病 空间学习记忆 八臂迷宫 

分 类 号：R686[医药卫生—骨科学]