HPLC法测定胃力片中大黄酸、大黄素、大黄酚和大黄素甲醚的含量  

Determination of rhein,emodin,chrysophanol and rheochrysidin in Weili tablet by HPLC

作　　者：张红霞[1] 金艺[1] 许海燕[1] 赵怀清[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2007年第7期417-420,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立胃力片中大黄酸、大黄素、大黄酚、大黄素甲醚的含量测定方法。方法采用HPLC法,色谱柱：Diamonsil C18（4.6 mm×200 mm,5μm）,以甲醇-体积分数为0.1%的磷酸水溶液（体积比为82∶18）为流动相,流速：1.0 mL·min^-1,柱温：35℃,检测波长：254 nm。结果大黄酸、大黄素、大黄酚、大黄素甲醚分别在1.42-12.79、2.02-18.14、1.28-11.52、0.76-8.36 mg·L^-1内呈良好的线性关系,相关系数分别为0.999 1、0.999 4、0.999 0和0.999 6,平均回收率分别为102.7%（RSD=1.0%,n=9）、101.2%（RSD=2.7%,n=9）、99.4%（RSD=1.6%,n=9）和97.9%（RSD=2.8%,n=9）。结论本方法可作为胃力片质量控制方法之一。Objective To develop a HPLC method for determination of rhein, emodin, chrysophanol and rheochrysidin in Weili tablet （traditional Chinese medicines）. Methods The analysis was carried out with a Diamonsil C18（4.6 mm× 200 mm, 5 μm） column and a mobile phase of methanal - 0.1% phosphoric acid solution（ V： V = 82：18）. The flow rate was 1.0 mL·min^-1 and column temperature was 35℃ .The detection wavelength was set at 254 nm. Results The calibration curve was linear within the range of 1.42 - 12.79 mg·L^-1,2.02- 18.14 mg·L^-1, 1.28 - 11.52 mg·L^-1 and 0.76 - 8.36 mg·L^-1 for rhein, emodin, chrysophanol and rheochrysidin, respectively. The average recoveries were 102.7 % , 101.2 %, 99.4 % and 97.9 % for rhein, emodin, chrysophanol and rheochrysidin, respectively. Conclusion This method is convenient, accurate and reproducible for determination of rhein, emodin, chrysophanol and rheochrysidin in Weili tablet.

关 键 词：胃力片 大黄酸 大黄素 大黄酚 大黄素甲醚 高效液相色谱法 

分 类 号：R917[医药卫生—药物分析学]