全反式维甲酸联合细胞因子诱导骨髓间充质干细胞分化为神经元样细胞的作用  

Differentiation of bone marrow mesenchymal stem cells into neurons-like cell use ATRA combined with cell factors in vitro

作　　者：陆华[1] 惠国桢[1] 苗宗宁[2] 吴卫江[2] 蒋云召[2] 周建宏[2] 陆爻忠[2] 陈革[2] 葛风[2] 徐杰[2] 

机构地区：[1]苏州大学附属第一医院神经外科,215006 [2]无锡市第三人民医院神经外科

出　　处：《江苏医药》2007年第7期701-703,F0002,共4页Jiangsu Medical Journal

基　　金：国家自然科学基金项目(30271325);江苏省自然科学基金项目(BK2001170)

摘　　要：目的探索全反式维甲酸(ATRA)、碱性成纤维细胞生长因子(bFGF)、表皮生长因子(EGF)在诱导骨髓间充质干细胞(BMSCs)分化为神经元样细胞过程中的作用。方法应用Ficoll分离骨髓中单核细胞,贴壁培养,观测形态并用流式细胞仪检测其细胞表面标志;用含ATRA、bFGF、EGF的条件培养基对培养的细胞向神经细胞诱导分化;采用免疫荧光染色法对分化的细胞进行鉴定。结果分离培养的贴壁细胞具有典型的BMSCs形态和细胞表面标志,诱导后细胞表达神经元和星形胶质细胞标志神经元特异性烯醇化酶(NSE)和胶质纤维酸性蛋白(GFAP)。结论ATRA联合细胞因子具有诱导BMSCs在体外分化为神经元样细胞的能力。Objective To investigate the effect of basic fibroblast growth factor （ATRA）, combined with（b-FGF） and epidermal growth factor（EGF） on inducing bone marrow mesenchymal stem cells（BMSCs） into neurons-type cell in vitro. Methods The monocyte was isolated from bone marrow with Ficoll,cultured and examined for morphology and surface markers. At last the cultured cells were induced to differetiate into neurowlike cells by ATRA, b-FGF and EGF, and the differentiated cells were identified with irnmunofluorescence. Results The cultured cells had the typical morphology and cell surface antigen of BMSCs and could be induced to differetiate into neuron- like cells, exhibiting the neuonral phenotype, expressing neuron specificenolase（NSE）, and glial fibrillary acid protien（GFAP）. Conclusion ATRA combined with b-FGF and EGF can promote the differentiation of BMSCs into neuron-like cells.

关 键 词：全反式维甲酸 细胞因子 骨髓间充质干细胞 分化 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]