硝酸甘油及前列腺素对离体兔肺的保护作用  

The protective effect of PGE and glyceryltrinitrate perfsuion on the isolated lung

作　　者：周悦[1] 骆金华[1] 徐骁晗[1] 朱秉智[1] 

机构地区：[1]南京医科大学第一附属医院心胸外科,210029

出　　处：《江苏医药》2007年第7期706-708,共3页Jiangsu Medical Journal

基　　金：江苏省自然科学基金资助项目(537DA0302)

摘　　要：目的探讨硝酸甘油及前列腺素对离体兔肺的保护作用。方法18只兔随机分为对照组(A组)、硝酸甘油组(B组)和前列腺素E组(C组),每组6只。建立离体兔肺灌注模型。分别在2h和4h取肺部分标本做电镜分析。4h末检测肺湿/干重比(W/D)、肺气道压力(Paw)、并将自身静脉血用泵从主肺动脉泵入,用负压吸引真空试管将肺静脉血自左心房引出后做血气分析。结果C组肺组织气体交换功能的保护作用以及降低供体肺保存过程中含水量的作用明显优于其他组;降低气道压力和B组相当,明显优于对照组。B组肺实质细胞的气体交换功能的保护、降低肺含水量以及降低气道压力明显优于A组。结论UW液中加入前列腺素E进行离体肺灌注可以使肺保存时间明显延长、肺气体交换功能保存良好。Objective To investigate the protective effect of PEG1 and glyceryltrinitrate perfusion on the isolated lung. Methods Eighteen rabbits were randomly divided into 3 groups with 6 rabbits each. After the isolated lung model was established, the rabbits in group A were perfused with low temperature（4℃ ） UW solution as the control, the rabbits in group B and C with glyceryltrinitrate and DGE instead of UW, respectively. The lung specimens were obtained for analyzing morphologically at 2 and 4 hours after perfusion. The measurements included the ratio of wet/dry of weight （W/D）, airway pressure（Paw） of the lung model and gas analysis of the left atrial blood at 4 hours of perfusion- Results The protective effects on the function of the lung tissue gas exchange and the moisture capacity of group C were better than those of group A and B significantly. The protective effect of group B on Paw, moisture capacity and the function of lung tissue gas exchange was better than that of group A. Conclusion Perfusion of the isolated lung with PGE1 can get longer time of preservation and better preservation of the function of lung tissue gas exchange than that with glyceryltrinitrate or UW solution.

关 键 词：肺灌注 肺保护 前列腺素E 硝酸甘油 

分 类 号：R617[医药卫生—外科学]