细粒棘球蚴病患者淋巴细胞及细胞因子变化的初步观察  

Study on the Variation of Lymphocytes and Cytokines in Patients with Echinococcosis granulosus

作　　者：张琰 温浩 林仁勇 卢晓梅 张雪 冯晓辉 赵晋明[2] 张静萍 

机构地区：[1]新疆包虫病重点实验室,乌鲁木齐830054 [2]新疆医科大学第一附属医院普外三科,乌鲁木齐830054

出　　处：《中国寄生虫学与寄生虫病杂志》2007年第3期209-212,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金(No.30560140)~~

摘　　要：目的探讨细粒棘球蚴感染后机体淋巴细胞及细胞因子的变化。方法细粒棘球蚴病患者第1次手术组80例(汉族60,维族20例),第2次手术组37例(汉族24,维族13例),分别于术前采集外周血进行荧光抗体标记、流式细胞仪检测T细胞亚群、B细胞、自然杀伤细胞(NK细胞)及胞浆内细胞因子γ干扰素(IFN-γ)和白细胞介素4(IL-4)水平。选取179名健康成人为对照组。结果汉族患者第1次手术组的总T细胞(CD3+)低于对照组(P<0.05),第2次手术组的CD3+水平与对照组之间差异无统计学意义(P>0.05);辅助性T细胞(CD3+/CD4+)、NK细胞(CD3+/CD16,56+)和B细胞(CD3-/CD19+)水平,第1次手术组和第2次手术组的均显著低于对照组(P<0.01),细胞毒性T细胞(CD3+/CD8+)高于对照组(P<0.05)。维族患者第1次手术组的B细胞低于对照组(P<0.05);NK细胞水平第1次手术组和第2次手术组均低于对照组(P<0.01和P<0.05);3组间Th0和Th1差异无统计学意义(P>0.05),但第1次手术组Th2水平显著高于对照组(P<0.01)。结论感染细粒棘球蚴的患者机体免疫呈抑制状态;Th1、Th2水平在1次手术组合2次手术组间有所不同。Objective To investigate the variation of lymphocytes and cytokines in patients with cystic echinococcosis （CE）. Methods 80 CE patients who were diagnosed for the first time （60 of Han and 20 of Uygur nationality）, and 37 patients who were to accept the second surgical operation（24 Han and 13 Uygur nationality） were included in the study. The peripheral lymphocytes of patients before operation were analyzed by flow cytometry （FCM） to detect T and B lymphocytes, NK cells bearing surface markers, as well as Thl cytokine IFN-γ and Th2 cytokine IL-4 in the cytoplasm of lymphocytes. 179 healthy persons served as control. Results In the group of Han patients who were diagnosed for the first time, the percentage of total T cells（CD3^＋） was lower than the control （P〈0.05）, while among patients accepting the second operation, the ratio of total T cells showed no difference to the control. For the helper T cells （CD3^＋/CD4^＋）, NK cells （CD3^＋/CD16,56^＋） and B cells （CD3^-/CD19^＋）, their ratio were significantly lower in both groups than the control （P〈0.01）, but their cytotoxic T cells （CD3^＋/CD8^＋） were higher than the control. In Uygur patients diagnosed for the first time, B cell ratio was lower than that in control （P〈0.05）. The NK cell level in both groups of patients was lower than control （P〈0.01 and P〈0.05 respectively）. The level of Th0 and Thl showed no statistical difference among the three groups （P〉0.05）. The Th2 level was significantly higher in the first diagnosed patients than control （P〈0.01）, hut no statistical difference between the group with the second operation and the other two groups Conclusions The immune status of echinococcosis patients is inhibited and the level of Th1 and Th2 shows difference in the first and second operation groups.

关 键 词：细粒棘球蚴病 淋巴细胞免疫表型 流式细胞术 

分 类 号：R532.32[医药卫生—内科学]