依维菌素的高效液相色谱分析  

HPLC Analysis of Ivermectin

作　　者：吴春先[1] 王广成[1] 高立明[1] 陈丙坤[1] 聂果[1] 张忠明[1] 

机构地区：[1]四川省农药检定所,成都610041

出　　处：《农药》2007年第7期471-472,474,共3页Agrochemicals

摘　　要：采用高效液相色谱法分析依维菌素。使用C18反相柱和二极管阵列检测器,以甲醇-水(体积比82∶18)为流动相,柱温30℃,流速:1.0mL/min;检测波长244nm,外标法对依维菌素的有效成分进行定量分析。结果表明依维菌素的线性关系系数为0.9998,测定0.5%依维菌素乳油时,标准偏差为0.0089,变异系数为1.73%,平均回收率为100.14%;测定依维菌素原药时,标准偏差为0.10,变异系数为0.11%,平均回收率为99.88%。A HPLC method using C18 reverse phase column, a diode array detector and an external standard was used to quantitatively analyze the active ingredient of ivermectin. The mobile phase was methanol ＋ water（82：18, by volume）. Column temperature was 30 ℃, flow rate was 1.0 mL/min, and detection wavelength was 244 nm. The results showed that the linear correlation coefficient was 0.9998, the standard deviation was 0.0089, the variation coefficient was 1.73%, the average recovery was 100.14% when ivermectin 0.5% EC was detected, the standard deviation was 0.10, the variation coefficient was 0.11%, the average recovery was 99.88% when ivermectin TC was detected.

关 键 词：依维菌素 高效液相色谱 分析 

分 类 号：TQ450.7[化学工程—农药化工]