油菜抗菌核病的部分生理生化机制  

Partial biochemical and physiological mechanisms of Brassica napus mediating resistance to Sclerotinia sclerotiorum

作　　者：张志元[1] 张翼[2] 罗永兰[1] 官春云[3] 

机构地区：[1]长沙学院生物工程与环境科学系,长沙410003 [2]复旦大学生命学院,上海200433 [3]湖南农业大学油料作物研究所,长沙410128

出　　处：《中国油料作物学报》2007年第2期189-194,共6页Chinese Journal of Oil Crop Sciences

基　　金：国家"863"项目(2001AA241101)

摘　　要：矿质元素、单宁、内源草酸含量,超氧化物歧化酶(SOD)的活性及呼吸强度均与油菜品种(系)的抗病性有关。三个抗病品种(M004、中R-888、085)组织中的钙含量均高于中感品种甘油5号和高感病品种98C 40;高抗品种中R-888比中感品种甘油5号植株组织中的钙含量高16.4%。油菜植株组织中的铁含量高,感病性强,而锰、锌、铜的含量与抗菌核病没有相关性。各供试品种接种处理比对照(不接种)的单宁含量高;无论对照还是处理,抗性强的品种单宁含量均高于高感品种(98C 40)。丙二醛的含量与油菜对菌核病的抗病性没有明显的关系。油菜植株的内源草酸含量与品种的抗病性有关,高抗品种比感病对照,其内源草酸含量高22.2%。苯丙氨酸解氨酶(PAL)酶活性品种间无显著差异。SOD酶酶活性与抗病性的关系,不同类型的品种在接种菌核菌后,叶片中SOD活性变化不同,感病品种受到菌核菌侵染后,叶片中SOD酶活性增加值大于抗病品种。呼吸强度与抗病性的关系,接种后呼吸强度下降快的品种抗性强。The correlation between mineral elements content, tannin, endogenous oxalic acid content, SOD activity and resistance to Sclerotinia sclerotiorum in Brassica napus was studied. The calcium content in three resistant genotypes M004, ZhongR-888,085, was higher than that in susceptible ones Ganyou5 and 98C40. In the tissue of ZhongR-888, the content was 16.4% higher than that of Ganyou5. The higher content of iron, the more susceptibility of B. napus to S. sclerotioum was. While manganese, zinc, copper contents were not obviously related to resistance to S. sclerotioum. The tannin contents in inoculated varieties were higher than those in non-inoculated varieties. The tannin contents of resistant varieties with or without inoculation were higher than that of susceptible variety 98C40. The malonic aldehyde content did not show obvious relation with resistance. In the tissues of higher resistance variety（M004） oxalic acid content was 22. 2% higher than that of susceptible variety （98C40）. The PAL activity did not show remarkable differences among different varieties. After inoculation with S. sclerotioum, the SOD activity varied remarkably. The increase of SOD activity in susceptible varieties was greater than that of the resistant varieties. Those with rapid drop in respiration intensity after inoculation had higher resistance.

关 键 词：油菜 菌核病 抗病生理 

分 类 号：S435.645[农业科学—农业昆虫与害虫防治]