花生锈病抗性的AFLP标记  

Identification of AFLP markers for resistance to peanut rust

作　　者：侯慧敏[1] 廖伯寿[1] 雷永[1] 任小平[1] 王圣玉[1] 李栋[1] 姜慧芳[1] 黄家权[1] 陈本银[1] 

机构地区：[1]中国农业科学院油料作物研究所,湖北武汉430062

出　　处：《中国油料作物学报》2007年第2期195-198,共4页Chinese Journal of Oil Crop Sciences

基　　金：国家重大基础研究前期研究专项(2005CCA01900)

摘　　要：利用抗、感锈病花生品种为亲本配制杂交组合远杂9102×ICGV 86699,以其F2分离群体为研究材料,利用BSA法和AFLP技术,获得了与锈病抗性连锁的2个分子标记,与抗性基因间的遗传距离分别为10.90cM和7.86cM。利用获得的分子标记对其F3进行了验证,分子标记与抗性鉴定结果具有较高的一致性。An F2segregating population derived from Yuanza 9102 × ICGV86699 was used to identify DNA markers for resistance to rust in peanut by the AFLP approach. Based on genotyping and resistance testing in F2and F3generations,two markers linked with the resistance to rust were identified. The genetic distance of the two markers,M3L3-460 and M8L8-645, was 10. 9cm and 7.86cm respectively. Forty-seven plants in F3 generation were used to verify the reliability of the markers,and it was found that the DNA bands in the selected plants were highly correlated with the resistance performance.

关 键 词：花生 锈病 AFLP 分子标记 

分 类 号：S435.652[农业科学—农业昆虫与害虫防治]