基于硫堇/碳纳米管修饰金电极的过氧化氢生物传感器  

Hydrogen Peroxide Biosensor Based on Horseradish Peroxidase/Thionine/Carbon Nanotube Modified Glod Electrode

作　　者：欧朝凤[1] 袁若[1] 柴雅琴[1] 钟霞[1] 唐明宇[1] 陈时洪[1] 贺秀兰[1] 

机构地区：[1]西南大学化学化工学院重庆市现代分析化学重点实验室,重庆400715

出　　处：《分析化学》2007年第7期1011-1014,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No20675064);重庆市自然科学基金(NoCSTC-2004BB4149;2005BB4100);西南大学博士基金(NoswnvB2004021);西南大学高新技术培养基金(XSGX02)资助项目

摘　　要：制备了以硫堇（TH）、纳米金（Nano-Au）及多壁碳纳米管（MWNT）修饰的H2O2生物传感器。探讨了工作电位、温度、pH对电极响应的影响，考察了电极的重现性、抗干扰能力及使用寿命。该传感器具有线性范围宽、检出限低、灵敏度高、稳定性好和抗干扰能力强等特点。其线性范围为7．0×10^-7～4．0×10^-3mol／L：检出限为2．3×10^-7mol／L；灵敏度为0．13A／（mol L^-1cm^2）；达到稳定电流所用时间〈9s.米氏常数为0．62mmol／L，表明所固定的酶具有较高的生物活性。Horseradish peroxidase（HRP） was selected as a model enzyme to fabricate a novel biosensor based on the multi-walled carbon nanotube, thionine and nano-Au. The presence of nano-Au and multi-walled carbon nanotube in the film can provide suitable microenvironment for HRP and also efficiently retain its activity. The fabricated H2O2 sensor presents a linear range from 7.0 × 10^-7 to 4.0 × 10^ -3 mol/L（ H2O2 ）. The response time of stable current was less 9 s and the detection limit was 2.3 × 10^-7 moL/L. The apparent Michaelis-Menten constant （Km^app） for the sensor was 0.62 mmol/L and the sensitivity is 0.13 A/（ mol L^-1 cm^2 ）

关 键 词：硫堇 多壁碳纳米管 辣根过氧化物酶 纳米金 化学修饰电极 

分 类 号：O657.1[理学—分析化学]