雌二醇诱导小鼠胸腺细胞程序性死亡  

ESTRADIOL INDUCES APOPTOSIS IN MOUSE THYMOCYTES

作　　者：张修武[1] 牛喜林[1] 郭兆贵[1] 

机构地区：[1]湖南医科大学分子药理研究室

出　　处：《中国免疫学杂志》1997年第2期65-67,共3页Chinese Journal of Immunology

摘　　要：研究雌二醇对培养小鼠胸腺细胞的影响，发现用１μｍｏｌ／Ｌ、１００ｎｍｏｌ／Ｌ和１０ｎｍｏｌ／Ｌ１７β－雌二醇处理胸腺细胞２ｈ能诱导胸腺细胞产生典型的程序性死亡形态学改变和特征性ＤＮＡ“梯形”结构，并具有剂量依赖性。１０μｍｏｌ／Ｌ的它莫西芬可有效抑制１μｍｏｌ／Ｌ１７β－雌二醇诱导的胸腺细胞程序性死亡。同时，Ｓｔａｕｒｏｓｐｏｒｉｎｅ和放线菌酮亦能抑制雌二醇诱导的胸腺细胞程序性死亡。To investigate whether estradiol might induce apoptosis in mouse thymocytes.The mouse thymocytes were isolated and incubated in culture medium containing 17 β estradiol,17 β estradiol and tamoxifen,or staurosporine,or cycloheximide.DNA fragmentation was visualized by agarose gel electrophoresis.Estradiol elicited typical apoptosis morphological changes and DNA fragmentation in dose dependent manner.The results show that 1 μmol/L,100 nmol/L,10 nmol/L estradiol incubated with thymocytes for 2 h can induce a typical DNA ladder;10 μmol/L tamoxifen could inhibit apoptosis induced by 1 μmol/L estradiol in thymocytes;20 nmol/L staurosporine and 1 mg/L cycloheximide could inhibit the DNA fragmentation.In conclusion,estradiol is able to induce apoptosis in mouse thymocytes and tamoxifen could inhibit it.Protein kinase C and protein synthesis is involved in this event.

关 键 词：雌二醇 它莫西芬 胸腺细胞 细胞凋亡 

分 类 号：R392.12[医药卫生—免疫学] R977.9[医药卫生—基础医学]