促甲状腺素释放激素对小鼠脾细胞产生细胞因子的影响  

EFFECT OF THYROTROPIN RELEASING HORMONE ON THE PRODUCTION OF CYTOKINES IN MURINE SPLEEN CELLS

作　　者：陈其御[1] 陈京[1] 米从明[1] 

机构地区：[1]皖南医学院微生物学教研室

出　　处：《中国免疫学杂志》1997年第2期97-100,共4页Chinese Journal of Immunology

基　　金：安徽省卫生厅医学重点科研课题

摘　　要：观察促甲状腺素释放激素（ＴＲＨ）在体内和体外对小鼠脾细胞产生ＩＦＮ和ＩＬ－２的影响。结果表明，正常小鼠每天腹腔注射ＴＲＨ０．２μｇ／只，共１０ｄ，其脾细胞受ＣｏｎＡ刺激产生的ＩＦＮ和ＩＬ－２产量无显著增高（Ｐ＞０．０５）；对氢化可的松处理的免疫抑制小鼠，加用以上剂量ＴＲＨ后，其脾细胞产生ＩＦＮ和ＩＬ－２的能力明显提高（Ｐ＜０．０１）。在体外用ＴＲＨ孵育小鼠脾细胞，其浓度在０．００３９～１（１０－１０ｍｏｌ／Ｌ）时可显著提高ＣｏｎＡ诱生的脾细胞ＩＦＮ和ＩＬ－２的产量，且产量与ＴＲＨ的浓度呈正相关；但高于此浓度时，其产量与ＴＲＨ浓度呈负相关，呈现与剂量相关的双相调节作用。提示ＴＲＨ的免疫调节机理之一是通过影响这些细胞因子的产生。Studied the effect of thyrotropin releasing hormone(TRH) on the production of interferon(IFN) and interleukin 2(IL 2) induced by concanavalin A(ConA) in murine spleen cells in vivo and in vitro.The results showed that the products of IFN and IL 2 increased nonsignificantly(P>0 05),which induced by ConA in spleen cells of the normal mice treated I.P. with TRH of 0.2 μg a day for ten days,but the immunosuppressed mice after treatment with hydrocortisone were injected the same dose of TRH,in which the ability of spleen cells to product IFN and IL 2 rose notably(P<0 01).In vitro cultured the murine spleen cells with TRH and found that TRH at the concentration of 0.0039～1(10 -10 mol/L)significantly enhanced the products of IFN and IL 2,and there was a positive correlation between the products and TRH dosage,but TRH at the concentration beyond 1×10 -10 mol/L remarkably reduced the products of IFN and IL 2,and a negative dose effect relationship was obtained.It indicated that TRH has doublephase regulatory effect on the production of these cytokines in murine spleen cells.Thus,it suggest that one of the immunoregulative mechanisms of TRH seems to effect the production of cytokines.

关 键 词：促甲状腺素 释放激素 干扰素 白细胞介素2 TRH 

分 类 号：R392.12[医药卫生—免疫学] R977.14[医药卫生—基础医学]