一种新型的定量测定特异性体液免疫功能的溶血空斑试验  

AN NEW HEMOLYTIC PLAQUE TEST FOR QUANTITATIVLY DETECTING SPECIFIC HUMORAL IMMUNE FUNCTION

作　　者：安云庆[1,2] 辛永梅[1,2] 李玉兰[1,2] 李郁英 程松高[1,2] 

机构地区：[1]首都医科大学微生物免疫学教研室 [2]北京佑安医院中心实验室

出　　处：《中国免疫学杂志》1997年第2期101-103,共3页Chinese Journal of Immunology

摘　　要：根据补体经典途径激活时，可通过“旁立损伤”机制使邻近正常细胞溶解破坏的原理，该种溶血空斑试验不仅可以用来定量检测分泌ＩｇＭ的抗体形成细胞，也可直接用来定量检测分泌ＩｇＧ的抗体形成细胞。与传统溶血空斑试验相比，其最大的优点是可用来检测机体对任何一种可溶性抗原的特异性体液免疫应答能力，从而扩展了应用范围。The novel hemolytic plaque test was established on the basis that normal cells could be destroyed by “bystander damage” mechanism.When complement system was activated by classical pathway.The test not only can be used to detect the IgM secreting antibody producing cell,but also can be used to detect the IgG secreting antibody producing cell directly.The most benefit is that the novel hemolytic plaque test can be used to examine the specific humoral immune fuction of the body to any soluble antigens.Thus,its usage is greatly amplified.

关 键 词：溶血空斑试验 补体 体液免疫 免疫功能 

分 类 号：R446.63[医药卫生—诊断学]