表达谱芯片实验设计的比较分析  

Analysis on cDNA microarry experimental designs

作　　者：王洪宝[1] 李辉[1] 王启贵[1] 王宇祥[1] 

机构地区：[1]东北农业大学动物科学技术学院,黑龙江哈尔滨150030

出　　处：《东北农业大学学报》2007年第1期50-53,共4页Journal of Northeast Agricultural University

基　　金：国家自然科学基金重点项目(30430510);教育部优秀青年教师资助计划项目

摘　　要：通过两类表达谱芯片实验设计(包括直接杂交与间接杂交,正反标记试验),比较和分析了不同实验设计对实验结果的影响。结果表明,间接杂交的Log(Ratio)值的变化幅度要比直接杂交的大,主要原因是因为其实验系统误差要高于直接杂交;间接杂交的正反标记结果高度一致,说明间接杂交具有很高准确性,同时也表明荧光染料对实验结果无明显影响。Two groups of cDNA microarry experiments including direct and indirect hybridizing, and reciprocal crosses labeling were designed in order to compare and analyze the differences.The results revealed that the Log （Ratio） range in indirect hybridizing was wider than that in direct hybridizing, The major reason maybe that the systemic errors in indirect hybridizing are more excessive than direct hybridizing. The reciprocal crosses labeling experiments showed high consistency which suggests the indirect hybridizing is accurate, and fluorescent dyes made few effects on the results.

关 键 词：表达谱芯片 直接杂交 间接杂交 正反标记 

分 类 号：S565.1[农业科学—作物学] Q781[生物学—分子生物学]