PCR指纹法及PCR/SSO技术用于骨髓移植供者选择的比较研究  

PCR/FINGER PRINTING AND PCR/SSOP FOR SELECTION OF HLA MATCHED BONE MARROW DONORS

作　　者：王伟[1] 杨立[1] 田丁[1] 赫翠珍[2] 张权庚[2] 高天祥[2] 

机构地区：[1]首都医科大学宣武医院血液科 [2]首都医科大学实验中心

出　　处：《中国免疫学杂志》1997年第2期116-118,共3页Chinese Journal of Immunology

摘　　要：在１１个家系内进行了骨髓移植的配型工作。用ＰＣＲ分别扩增了供受者最具多态性的ＤＲＢ和ＤＱＢ基因的第二外显子，这些ＰＣＲ产物在非变性的聚丙烯酰胺凝胶电泳上显示具有单倍型特异性的，各不相同的带型格局，称为ＰＣＲ指纹。同时进行了ＰＣＲ指纹的交叉配型，及ＤＲＢ１和ＤＱＢ１基因的ＰＣＲ／ＳＳＯ杂交。ＰＣＲ指纹和交叉配型具有简单、经济、灵敏、准确、快速等优点，对骨髓移植供者的选择，有突出的优越性。Tissue typing for identification of HLA was carrried out among 11 patients,their siblings and parents.Besides the traditionally HLA typing by serology and mixed lymphocyte culture(MLC),have assessed a new DNA technique for testing DRB and DQB match between patient and their marrow donors called “PCR finger printing”.Meanwhile,hybridization of PCR products with SSOPs was also performed to confirm the match.Comparing with the other two methods for HLA classⅡ genotypig,PCR/SSO and PCR/RFLP,PCR finger printing is more rapid with results being available within 1 day,and more economic,because radioisotopes,southern blotting,restriction enzymes,and oligonucleotide probes are all unnecessary.It is also easy to perform and sensitive,and when carefully operated,accurate.

关 键 词：骨髓移植 白细胞抗原 聚合酶链反应 移植免疫学 

分 类 号：R457.7[医药卫生—治疗学] R392.4[医药卫生—临床医学]