金纳米粒子修饰石墨电极上鲁米诺-过氧化氢体系电化学发光行为的研究  

Electrogenerated Chemiluminesence of Luminal-Hydrogen Peroxide in Neutral PBS at a Graphite Electrode Modified With Gold Nanoparticles

作　　者：苗力孝[1] 漆红兰[2] 

机构地区：[1]延安大学化学与化工学院,陕西延安716000 [2]陕西师范大学化学与材料科学学院,陕西西安710062

出　　处：《延安大学学报（自然科学版）》2007年第2期66-68,72,共4页Journal of Yan'an University：Natural Science Edition

摘　　要：研究了磷酸盐缓冲溶液(pH=7.4)中金纳米粒子修饰石墨电极上鲁米诺-过氧化氢体系的电化学发光行为。实验结果表明在金纳米粒子修饰石墨电极上鲁米诺-过氧化氢的电化学发光强度增强近10倍,初步探讨了在金纳米粒子修饰石墨电极上鲁米诺-过氧化氢体系的电化学发光增强机理。在优化的实验条件下,鲁米诺浓度在4.0×10-12～1.0×1010-mol/L之间与电化学发光强度成良好的线性关系,方法的检出限为2×10-12mol/L,对5.0×10-11mol/L鲁米诺进行11次平行测定,相对标准偏差为5.3%。In this paper, the electrogenerated chemiluminescence （ECL） behavior of luminol-hydrogen peroxide at a graphite electrode modified with gold nanoparticles （ GN-GE ） in 0.10 M phosphate buffer solution （ pH 7.4, containing 0.10 M KCl） was investigated. When an applied potential was scanned from 0 to 0.85 V, an ECL intensity of luminol-hydrogen peroxide at GN-GE about 0.65 V were increased about ten times compared with that of at bare GE. The ECL mechanism of luminol-hydrogen peroxide at GN-GE was investigated. Based on the increased ECL, a highly sensitive ECL method for the determination of luminol has been developed at the GN-GE. Under the optimized conditions, the calibration graph of the ECL emission intensity vs. luminol concentration was linear from 4. 0 × 10^-12 to 1.0 × 10^-10mol/L. The detection limit of luminol is 2 × 10^-12mol/L and relative standard derivative was 5.3% for 5.0 × 10^-11mol/L luminol （ n = 11 ）.

关 键 词：纳米金粒子 石墨电极 鲁米诺 过氧化氢 电化学发光 

分 类 号：O657.7[理学—分析化学]