血小板激活因子在急性重症胰腺炎发病过程中作用的实验研究  

The role of platelet activating factor in pathogenesis of acute pancreatitis in dogs

作　　者：冀振华[1,2,3] 王本茂[1,2,3] 李少华 唐岩[1,2,3] 丁廷楷 马元桂[1,2,3] 

机构地区：[1]海军总医院肝胆外科 [2]上海第二军医大学长海医院普外科 [3]海军医学研究所航海医学研究室

出　　处：《中华外科杂志》1997年第2期108-110,共3页Chinese Journal of Surgery

摘　　要：作者为探讨血小板激活因子在狗急性重症胰腺炎发病过程中的作用，采用１７只比格（Ｂｅａ－ｇｌｅ）狗，随机分为三组：对照组（ｎ＝５）、胰腺炎组（ｎ＝６）、治疗组（ｎ＝６）。胰腺炎组及治疗组动物制成急性重症胰腺炎动物模型。治疗组动物在模型复制后５ｍｉｎ，３ｈ分别静脉注射血小板激活因子（ＰＡＦ）拮抗剂ＢＮ５２０２１（５ｍｇ／ｋｇ）。用温氏法测定血清淀粉酶活性，血小板聚集法测定血清及胰腺组织中ＰＡＦ的含量。结果显示：急性重症胰腺炎模型复制成功后３０ｍｉｎ，胰腺炎组血清淀粉酶活性即上升到基础值的２００±４４．７％，８ｈ高达４６６．７±１１１．６％。８ｈ时与对照组、治疗组差异均有显著性意义（Ｐ＜０．０５）。胰腺炎组血中ＰＡＦ含量在３０ｍｉｎ时即开始上升，８ｈ达最高１１．８１±０．７８ｎｇ／ｍｌ，在３０ｍｉｎ之后与对照组比较即差异有非常显著的意义（Ｐ＜０．０１），而１ｈ之后与治疗组差异有非常显著的意义（Ｐ＜０．０１）。胰腺组织中ＰＡＦ含量，胰腺炎组比治疗组及对照组明显增高（Ｐ＜０．０１）。作者认为ＰＡＦ在急性重症胰腺炎的发病过程中起重要作用，ＰＡＦ拮抗剂有希望成为治疗急性重症胰腺炎的有效药物。Beagle′s dogs were divided randomly into tree groups: pancreatitis group(PG, n =6),pan+BN52021 group(BNG, n =6),control group(CG, n =5).The acute pancreatitic model of PG and BNG was established by injecting sodium taurocholate and trypsin into the main pancreatic duct. Animals of BNG were injected PAF receptor antagonist BN52021 (5ml/kg) intravenously 5 minutes and 3 hours respectively after acute pancreatitis induction. Blood amylase activity was determined by Winslow′s method. PAF in blood and pancreatic tissue was determined by the platelet accumulation method.Blood amylase activity of PG increased by 466 7±111 6 than the baseline at 8 hours and increased significantly than that of BNG and CG ( Р <0 05).Blood PAF level of PG increased from 30 minutes to 11 81±0 78 ng/ml at 8 hours. BN52021 inhibited very significantly the increase of PAF level ( P <0 01).PAF level in pancreatic tissue of PG was significantly higher than that of BNG and CG ( P <0 01).PAF may play an important role in early acute pancreatitis.

关 键 词：胰腺炎 血小板活化因子 病理学 

分 类 号：R657.510.2[医药卫生—外科学]