大鼠胎脑组织移植免疫排斥反应的实验研究  

Immunological rejection in rat embryonic brain tissue transplantation: experimental study

作　　者：刘俊[1] 张可成[1] 

机构地区：[1]第三军医大学新桥医院神经外科,重庆630037

出　　处：《中华外科杂志》1997年第1期16-18,共3页Chinese Journal of Surgery

基　　金：全军"八五"攻关课题基金

摘　　要：以Wistar大鼠作为受体动物,采用延迟移植技术,将胎龄16～18天的同种及异种胎脑皮质组织移植到受体动物预制脑腔内。术后给予环孢霉素A治疗。移植后动物存活2周、4周及12周左右,分批处死采用ABC法进行免疫组化染色,了解移植区局部T淋巴细胞亚群(总T细胞、T_H细胞及Tc/s细胞)浸润情况及MHC Ⅱ类抗原的表达情况。结果显示:同种移植组存活率明显高于异种移植组。而同种移植治疗组存活率又明显高于同种移植未治疗组(P<0.01)。在移植区发现大量T_H细胞和Tc/s细胞表面抗原及MHC Ⅱ类抗原的表达,而正常脑组织免疫组化染色均为阴性反应。表明在移植区存在有排斥反应,脑组织不是免疫特免器官,应用免疫抑制剂可有效增加移植物存活率。The embryonic cerebral neocortex from rat or mouse donors which were 16 to 18-dayold embryos was graftd into the neocortex of adult recipient rats. One group was treated with cyclosporin A. At different time after transplantation, the brain graft tissues were stained immunocytochemically to exmine the expression of MHC-elass Ⅱ antigens and both subsets of T-cells, helper-in ducer and cytotoxic-suppressor T-cells. The results indicated that the survival rate of treated group was higher than that of non treated with immunosuppressant. Immunocytochemical evaluation showed that a large number of helper T-cells and cytotoxic T-cells appeared, with a significant increase of the number of class Ⅱ major histocompatibility complex(MHC) expressing cells within and around the allografts was obsvered. These MHC-class Ⅱ antigen-positive cells may be lymphocytes, microglia and astrocytes. We conclude that the CNS is not a 'immunologically privileged site' and there is graft rejection in brain transplantation. It is necessary for brain transplantation to be treated with immunosuppressant.

关 键 词：胎组织移植 移植排斥 T淋巴细胞 胎脑移植 

分 类 号：R651[医药卫生—外科学]