L-丙酰肉碱对再灌注心肌损伤的保护及体外抗自由基作用  

Protective effect of L-propionylcarnitine on myocardial injury induced by ischemia and reperfusion and its antiradical effects

作　　者：胡建强[1] 丁鸿钧[1] 秦永文[1] 章同华[1] 郑兴[1] 陈士明[2] 严小敏 

机构地区：[1]第二军医大学长海医院心血管内科,上海200433 [2]复旦大学分析测试中心

出　　处：《第二军医大学学报》1997年第1期41-43,共3页Academic Journal of Second Military Medical University

摘　　要：目的:观察L－丙酰肉碱对缺血再灌注心肌的保护作用，并探讨其机理。方法：采用大鼠在体心脏冠状动脉前降支阻断5min造成缺血再灌注模型，持续心电监护，观察心律失常与心功能指标；应用电子顺磁共振观察L－丙酰肉碱对Fenton体系及二甲基亚砜（DMSO）碱性有氧体系产生自由基的作用。结果：L－丙酰肉碱静脉给药明显减少再灌注时室性心动过速、室性纤维颤动的发生（P＜0．05），促进再灌注后左室收缩压、左室压最大上升速率、左室压最大下降速率、心力环面积的恢复，与对照组相比，各参数均明显增高（P＜0．05）；静脉注射L－丙酰肉碱100mmol／L时促进Fenton体系OH的产生，而500mmol／L时产生明显抑制作用，L－丙酰肉碱明显抑制DMSO碱性有氧体系OS的产生。结论：L－丙酰肉碱明显减少再灌注心律失常的发生，促进再灌注后心功能的恢复。推测它对自由基的作用可能是再灌注损伤保护机理之一。To investigate the protective effect of L-propionylcarnitine on reperfused myocardiumand the mechanism of its effect. Methods: 28 anesthetized open chest rats with LAD occlused 5 min andthen reperfused were divided into control and treatment groups. During reperfusion, the incidence of PVC,VT and VF was observed; ECG signal and left ventrical pressure (obtained by catheter) were analysed byMCCA-9 software, and the index of cardiac function was detected. Its effect on generation of free radicalsin Fenton system and alkaline DMSO system was detected by ESR. Results: Pretreatment with L-propi-onylcarnitine reduced the incidence of VT and VF (P<0. 05) compared with those of control. It also im-proved the recovery of LVSP, +P max, -P’ max and Lo. In treated rats, LVSP was -higher than that in con-trol at 5 min after reperfusi0n (P<0. 05); +P was higher at lO and 20 min (P<O. O5 ), P washigher at 20 min (P<0.05); Lo was higher at 5, 10 and 20 min(P<0. 05). The drug (100 mmol/L) in-creased the generation of OH in Fenton system, but at concentration 500 mmol/L, it significantly sup-pressed. L-propionylcarnitine (25 mmol/L) significantly suppressed the generation of O: in alkaline DM-SO system. Conclusion: L-propionylcarnitine improves the recovery of myocardial functlun during reperfu-sion, thus reducing the incidence of reperfusion-induced arrhythmia. Its effect 0n free radicals may be themechanism of its protective effects-

关 键 词：心肌缺血 再灌注损伤 心肌保护 L-丙酰肉碱 

分 类 号：R654.2[医药卫生—外科学]