云芝菌丝体液体培养基的筛选  

Selection of Liquid Culture Medium on Mycelium of Coriolus versicolor

作　　者：马海燕[1] 郭成金[1] 

机构地区：[1]天津师范大学化学与生命科学学院,天津300074

出　　处：《中国食用菌》2007年第4期34-36,共3页Edible Fungi of China

基　　金：天津市科学技术委员会项目(编号:06ZHCXNC05900)

摘　　要：以云芝菌丝体生物量为主要指标,首先采用不同碳源和氮源进行单因素初选试验,再进行其不同比例的混合碳氮源正交试验。结果表明云芝菌丝体液体最佳培养基是葡萄糖20g.L-1、蔗糖5g.L-1、酵母粉2g.L-1、蛋白胨2g.L-1、KH2PO42.5g.L-1、MgS04.7H2O1.5g.L-1和VB18mg.L-1。其菌丝体生物量得率22.9g.L-1。Biomass of mycelium in liquid culture medium for Coriolus versicolor as major index, firstly different carbon source and nitrogen source were adopted to carry out the single factor primary election in the experiment, carrying out tests again for different proportional commixture of carbon source and nitrogen source. The results showed that the best medium was glucose 20g/L, cane sugar 5g/L, yeast powder 2g/L, peptone2g/L, KH2PO4 2. 5g/L, MgSO4 · 7H2O 1.5g/L and VB1 8mg/L and the production rate of biomass on mycelium of Coriolus versicolor reached 22. 9g/L in the liquid culture medium.

关 键 词：云芝 液体培养基 筛选 菌丝体 生物量 

分 类 号：S567.31[农业科学—中草药栽培]