CLIA和ELISA检测婴儿肝病综合征患者人巨细胞病毒特异性IgM抗体的比较  

Detecting human cytomegalovirus-specific IgM antibody in infant hepatism syndrome by CLIA and ELISA assays

作　　者：吉耀华[1] 王岳平[1] 阮强[1] 王继东[1] 刘庆[1] 陈淑荣[1] 孙峥嵘[1] 何蓉[1] 齐莹[1] 马艳萍[1] 

机构地区：[1]中国医科大学附属盛京医院病毒实验室,辽宁沈阳110004

出　　处：《临床儿科杂志》2007年第7期532-533,536,共3页Journal of Clinical Pediatrics

摘　　要：目的评价LIAISON化学发光免疫分析法(CLIA)检测人巨细胞病毒(HCMV)特异性IgM抗体的方法学特点。方法采用CLIA法对婴儿肝病综合征患者血清进行HCMVIgM检测,同时用ELISA试验进行同一批样本中部分的测定。结果CLIA检测584例,阳性132例,阳性率22.6%。两种检测方法的一致性为88.8%,但CLIA法的敏感性和特异性均高于ELISA(P<0.05)。结论CLIA检测HCMVIgM具有灵敏度高、检测所需时间短、人为影响因素少、结果准确等优点,适用于临床检测。Objectives To evaluate the use of LIAISON chemiluminescent immunoassay （CLIA）in the detection of the human cytomegalovirus （HCMV）specific IgM antibody. Methods The specific HCMV-IgM antibodies in infants with hepatism syndrome were detected by CLIA and enzyme linked immunosorbent assay（ELISA）. Results Among 584 samples detected by CLIA, 132 （22.6%）were positive. The consistency of results between CLIA and ELISA was 88.8 % and CLIA was significantly more sensitive than ELISA（P 〈 0.05）. Conclusions Detection of HCMV-IgM by LIAISON CLIA is sensitive.time-saving.less affected by human factors and accurate. Therefore ,CLIA is suitable for clinical diagnosis.

关 键 词：婴儿肝病综合征 人巨细胞病毒 LIAISON化学发光免疫分析法 

分 类 号：R725.7[医药卫生—儿科]