隐孢子虫病两种诊断方法的应用比较  

DETECTION OF CRYPTESPORIDIUM INFECTION BY TWO METHODS

作　　者：徐梅倩[1] 黄侠[1] 朱顺海[1] 黄燕[1] 曹杰[1] 何国声[1] 

机构地区：[1]中国农业科学院上海兽医研究所,上海200232

出　　处：《中国兽医寄生虫病》2007年第4期F0002-F0002,F0003-F0004,共3页Chinese Journal of Veterinary Parasitology

基　　金：上海市科技兴农重点攻关项目(沪农科攻字2004-12-3)

摘　　要：目的对目前常用的两种检测隐孢子虫感染方法进行估价。方法取上海某牧场随机采集的20头奶牛粪便用改良抗酸染色法和巢式PCR(Nested PCR)法检测。结果两种方法均能检测出粪便中的隐孢子虫卵囊,改良抗酸染色法阳性检测率55%,Nested PCR法阳性检测率70%。结论:两种方法均能从感染隐孢子虫的奶牛粪便中捡出隐孢子虫卵囊,可根据需要选择。Objective To assess two methods for detection of Cryptesporidium infection. Methods Cryptesporidium in the feces of 20 milch cows from a cattle farm in Shanghai were detected by modified acid-fast staining and nested PCR. Results Two methods could detect Cyrptesporidium oocysts from random samples, and the positive rates of Cryptesporidium detected by modified acid-fase staining and nested PCR were 55% and 70% respectively. Conclusion Two methods could detect Cryptesporidium oocysts from the feces of milch cows infected Cryptesporidium, and could be chosen by different methods.

关 键 词：隐孢子虫 改良抗酸染色法 巢式PCR法 

分 类 号：S858.235.923[农业科学—临床兽医学]