应用壳核纳米微球固定乙醇脱氢酶联用HPLC对抑制剂筛选  

Application of Alcohol Dehydrogenase Immobilized on Core-shell Nanoparticles to Screening Inhibitors by HPLC

作　　者：杜志云[1] 潘文龙[2] 毛学圃[3] 黄宝华[1] 黄仲立[3] 方岩雄[1] 张坤[1] 马林[3] 

机构地区：[1]广东工业大学轻工化工学院,广东广州510006 [2]中国广州分析测试中心,广东广州510070 [3]中山大学化学化工学院,广东广州510275

出　　处：《分析测试学报》2007年第4期455-458,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金重点资助项目(20376015);广东省自然科学基金重点资助项目(2006B13081007)

摘　　要：对新型聚甲基丙烯酸甲酯/壳聚糖纳米微球进行制备、纯化和表征,采用该纳米微球对乙醇脱氢酶进行固定化。让多组分天然产物直接作用于固定化乙醇脱氢酶,通过高效液相色谱(HPLC)检测固定化酶吸附组分,研究多组分天然产物混合物对乙醇脱氢酶具有抑制活性的有效成分。结果表明:由HPLC检测到的被固定化乙醇脱氢酶从多组分化合物中直接吸附的组分对乙醇脱氢酶均有较强的抑制作用,说明该方法从多组分直接筛选抑制剂是可行的,同时,本文所检测到的7种天然化合物对乙醇脱氢酶的抑制活性还没有报道。Novel poly （methyl methacrylate）/chitosan core-shell nanoparticles were prepared, purified and characterized, and was applied to immobilize alcohol dehydrogenase. The mixture of multiple components of natural products was directly absorbed by the immobilized alcohol dehydrogenase （ IAD）, and the components absorbed were detected by HPLC. The inhibition of each component absorbed on IAD was determined. The result showed that the absorbed components detectable by HPLC exerted potent inhibitory activities. This indicated that the proposed method can be used to screen the inhibitors directly from the multiple component mixtures. Seven natural compounds detected as inhibitors of alcohol dehydrogenase were not reported before.

关 键 词：壳核纳米微球 乙醇脱氢酶 固定化 高效液相色谱 抑制剂 

分 类 号：O657.72[理学—分析化学] Q841.1[理学—化学]