牛奶中12种磺胺类药物残留的高效液相色谱测定方法  

Determination of 12 Sulfonamides in Milk by HPLC

作　　者：方炳虎[1] 何绮霞[2] 邹潍力[1] 陈建新[1] 邓国东 肖田安 吴秋豪 林海丹 

机构地区：[1]华南农业大学兽医学院,中国广州510642 [2]广东省农业科学院畜牧所,中国广州510642 [3]广东省兽药与饲料监察总所,中国广州510230

出　　处：《分析测试学报》2007年第4期519-522,共4页Journal of Instrumental Analysis

基　　金：广东省农业攻关计划(2005B20201011)

摘　　要：建立了高效液相色谱（HPLC）检测牛奶中12种磺胺类药物（磺胺二甲基嘧啶、磺胺间甲氧嘧啶、磺胺甲嗯唑、磺胺对甲氧嘧啶、磺胺喹嗯啉、磺胺氯哒嗪、磺胺氯吡嗪、磺胺嘧啶、磺胺噻唑、磺胺甲基嘧啶、磺胺甲噻二唑、磺胺地索辛）残留的方法，考察了样品的提取、净化及色谱分析条件。牛奶样经乙酸乙酯提取、固相萃取净化后上机分析，11种磺胺药物标准曲线在10～800μg／L，SM2在5-200μg／L质量浓度范围内相关系数r〉0.999，回收率为76％～106％，相对标准偏差小于15．9％。检出限为5-8μg／L，定量下限为14～27μg／L。A high-performance liquid chromatography method for simultaneous determination of sulfadiazine, sulfamethazine, sulfamethoxypyridazine, sulfamonomethoxine, sulfamethoxazole, sulfadimethoxine, sulfaquinoxaline, sulfadiazine, sulfathiazole, sulfamerazine, sulfamethizole and sulfadimethoxine in milk was described in this paper. The milk samples were extracted with ethyl acetate, and cleaned by a SXC column solid phase extraction（SPE）. The calibration curves for 12 sulfonamides were linear in a concentration range of 10-800μg/L（ SM2 5 -200μg/L） , with r 〉0. 999. The recoveries of the analytes in fortified samples ranged from 76% to 106% , with the RSD 〈 15.9%. The limits of detection were 5 -8 μg/L, and the limits of quantification were 14 -27 μg/L.

关 键 词：磺胺类药物 牛奶 固相萃取 高效液相色谱 

分 类 号：TQ116.2[化学工程—无机化工]