吖啶橙-罗丹明6G能量转移荧光猝灭法定量测定茶多酚  

Determination of Tea Polyphenols with Acridine Orange-Rhodamine 6G by Energy Transfer Fluorescence Quenching Method

作　　者：曹冬林[1] 刘保生[1] 傅丽[2] 锅圆[1] 

机构地区：[1]河北大学理化分析中心河北省分析科学技术重点实验室 [2]廊坊师范学院化学系,河北廊坊065000

出　　处：《分析测试学报》2007年第4期537-540,共4页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20075005)

摘　　要：在λex／λem=450／556nm，十二烷基苯磺酸钠（DBS）存在下，吖啶橙（AO）-罗丹明6G（R6G）间能够发生有效的能量转移，使R6G荧光大大增强。酸性条件下，茶多酚的加入使体系R6G的荧光发生猝灭。利用AO-R6G能量转移荧光猝灭法定量测定茶多酚，提高了测定的灵敏度和选择性。茶多酚含量在0．75-60mg／L范围内与R6G荧光猝灭程度呈良好线性关系，方法检出限为0．35mg／L。6次平行测定样品相对标准偏差1．36％-2．17％，回收率为93％～107％。该方法用于茶叶样品中茶多酚含量的测定。An effective energy transfer could occur between Acridine orange（AO） and Rhodamine 6G （R6G） at λex450 nm and λem556 nm in the presence of dodecyl benzene sodium sulfonate（DBS）, leading to significant increase of R6G fluorescence intensity; but in the presence of tea polyphenols and ammonium molybdate in 0.6 mot/L HCI solution, the R6G fluorescence intensity at λ556nm would be quenched to a certain degree according to the amount of tea polyphenols. Based on the above phenomena, a new method for the determination of tea polyphenols with the energy transfer fluorescence quenching of AO - R6G was developed. The linear range of the response for tea polyphenols was 0.75 - 60 mg/L with a detection limit of 0.35 mg/L. The relative standard deviation range was 1.36% - 2. 17% for 6 parallel determinations of tea polyphenols. The recovery range was 93% - 107%. The method has been applied to the determination of tea polyphenols in black tea and green tea in China.

关 键 词：吖啶橙 罗丹明6G 能量转移 荧光猝灭 茶多酚 

分 类 号：O657.3[理学—分析化学]