黄连解毒汤对2型糖尿病大鼠靶组织葡萄糖转运子4的影响  

Effects of Huanglian Jiedu Decoction on glucose transporter 4 in target tissues of type 2 diabetic rats

作　　者：陈广[1] 陆付耳[1] 徐丽君[1] 

机构地区：[1]华中科技大学同济医学院同济医院中西医结合研究所,湖北武汉430030

出　　处：《中西医结合学报》2007年第4期412-415,共4页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金资助项目(No.30371816);高等学校博士学科点专项科研基金资助课题(No.20030487008)

摘　　要：目的:观察黄连解毒汤对2型糖尿病大鼠脂肪和肌肉组织葡萄糖转运子4(glucose transporter4,GLUT4)蛋白表达及转位的影响,以此探讨黄连解毒汤治疗2型糖尿病的分子机制。方法:采用尾静脉注射小剂量(30mg/kg)链脲佐菌素(streptozotocin,STZ)加高脂高热量饮食喂养的方法建立2型糖尿病大鼠模型,将造模动物随机分为模型组、阿司匹林组和黄连解毒汤组,另设正常对照组。药物干预治疗10周后,各组动物进行口服糖耐量试验(oral glucose tolerance test,OGTT)并检测各组动物体质量变化、空腹血糖(fasting blood glucose,FBG)、血清胰岛素(fasting seruminsulin,FINS)及脂肪和肌肉组织胰岛素刺激前后GLUT4蛋白表达水平。结果:与模型组相比,黄连解毒汤组OGTT明显改善,大鼠体质量减轻,FBG降低,胰岛素刺激前后脂肪和肌肉组织中的GLUT4蛋白表达均较高。结论:黄连解毒汤对2型糖尿病的治疗效应可能与提高脂肪和肌肉组织中GLUT4的蛋白表达及转位有关。Objective： To investigate the molecular mechanism of Huanglian Jiedu Decoction （HLJDD）, a traditional Chinese compound herbal medicine, in treating type 2 diabetes mellitus （T2DM） by observing its effects on glucose transporter 4 （GLUT4） protein expression and translocation in adipose and skeletal muscle tissues of rats with T2DM. Methods： T2DM was induced in rats by intravenous injection of a small dose of streptozotocin （STZ, 30 mg/kg） plus high fat and high caloric laboratory chow. Then animals were divided into untreated group, aspirin-treated group and HLJDD-treated group. Normal rats fed with common chow were designated as normal control group. Ten weeks later, the oral glucose tolerance test （OGTT） was performed in all animals, and the changes of murine body weight, fasting blood glucose （FBG）, fasting serum insulin （FINS） and the expression of GLUT4 protein in skeletal muscle and adipose tissues before and after insulin treatment were routinely determined. Results： Compared with the untreated group, the result of OGTT of HLJDD-treated group was improved. The levels of the body weight and FBG were decreased, while the GLUT4 protein expression and translocation were elevated obviously. Conclusion： It is suggested by the present results that the therapeutic effects of HLJDD on T2DM might be related to its ability of increasing GLUT4 protein expression and translocation in adipose and skeletal muscle tissues of T2DM rats.

关 键 词：2型糖尿病 葡萄糖转运蛋白 胰岛素 空腹血糖 

分 类 号：R285[医药卫生—中药学]