黄芪多糖对3T3-L1前脂肪细胞增殖和分化的影响  

Effects of Astragalus polysaccharides on proliferation and differentiation of 3T3-L1 preadipocytes

作　　者：刘毅[1] 王文健[1] 陈伟华[1] 应健[1] 

机构地区：[1]复旦大学中西医结合研究所,上海200040

出　　处：《中西医结合学报》2007年第4期421-426,共6页Journal of Chinese Integrative Medicine

基　　金：教育部985工程资助项目(No.2001-13);国家自然科学基金资助项目(No.30672735);国家教委高校博士点基金资助项目(No.20050246041)

摘　　要：目的:探讨黄芪多糖(Astragalus polysaccharides,APS)对前脂肪细胞增殖、分化的影响及其机制。方法:以XTT法检测3T3-Ll前脂肪细胞的增殖;油红O染色并通过比色定量分析检测3T3-L1前脂肪细胞分化过程中胞浆脂质的堆积;采用实时聚合酶链式反应和蛋白质免疫印迹(Western blotting)技术检测前脂肪细胞分化相关基因过氧化物体增殖剂活化受体γ(peroxi some proliferator activated receptorγ,PPARγ)、CAAT/增强子结合蛋白α(CAAT/enhancer binding proteinα,C/EBPα) mRNA以及蛋白质的表达。结果:APS浓度从0.025到0.8g/L,在24、48和72h各时间段对前脂肪细胞的生长均表现为增殖趋势,且呈一定的量效关系;0.4g/L APS处理的前脂肪细胞,胞浆中出现较大量脂滴,其作用与噻唑烷二酮(thiazo-lidinedione,TZD)类药物罗格列酮(rosiglitazone,ROS)相似;APS使前脂肪细胞分化相关基因PPARγ和C/EBPα mRNA与蛋白的表达明显增加,与空白对照组相比,差异有统计学意义(P<0.05,P<0.01)。结论:APS能够促进前脂肪细胞的增殖和分化,增加脂肪细胞分化过程中脂质的堆积,其机制可能与其促进PPARγ和C/EBPα mRNA与蛋白质表达有关。Objective： To observe the effects of Astragalus polysaccharides （APS） on the proliferation and differentiation of 3T3-L1 preadipocytes and to elucidate its possible mechanism. Methods： The proliferation of 3T3-L1 preadipocytes was detected by XTT method. Lipid droplets accumulated in cytoplasm of the differentiated preadipocytes were observed by using red O staining and quantified by colorimetry. The expressions of peroxisome proliferation activated receptor γ （PPARγ） and CAAT/enhancer binding protein （C/EBPα） mRNAs and proteins were detected by real-time polymerase chain reaction and Western blotting respectively. Results： APS at different concentrations （0. 025-0. 8 g/L） affected 3T3-L1 preadipocyte proliferation and differentiation dose-dependently. 3T3-L1 preadipocytes treated with 0.4 g/L APS had lots of lipid droplets in the cytoplasma, which were similar to cells treated with rosiglitazone （ROS）. APS significantly increased the mRNA and protein expressions of PPARγ and C/EBPα （ P〈0.05, P〈0.01, compared with the normal control group） in the course of 3T3-L1 preadipocyte differentiation. Conclusion： APS can promote the proliferation of 3T3-L1 preadipocytes, enhance the accumulation of lipid drops, and increase the terminal differentiation of preadipocytes, which may be associated with its effects in increasing the expressions of PPARγ and C/EBPα mRNAs and proteins. The study suggests that APS has potential in the treatment of metabolic syndrome.

关 键 词：黄芪多糖 增殖 分化 MRNA 前脂肪细胞 

分 类 号：R285.5[医药卫生—中药学]