低温冰箱转液氮阶梯降温法冷冻保存自体外周血造血干细胞移植治疗恶性血液病的临床研究  

Treatment of hemotologic malignancies with autologous Hemopoietic stem cell transplantation cryopreserved by ladder-style freezing from low temperature refrigerator to liquid nitrogen

作　　者：杨同华[1] 赵仁彬[1] 沈晓梅[1] 史克倩[1] 杨艳梅[1] 赖洵[1] 

机构地区：[1]云南省第一人民医院血液科,云南昆明650032

出　　处：《中国实用内科杂志》2007年第14期1119-1121,共3页Chinese Journal of Practical Internal Medicine

摘　　要：目的探讨低温冰箱转液氮阶梯降温冷冻保存对自体外周血造血干细胞移植的影响。方法2001年4月至2006年4月对云南省第一人民医院血液科31例行自体外周血造血干细胞移植的患者随机分为程序降温转液氮法(15例)和低温冰箱转液氮阶梯降温法(16例)两组,比较两组患者造血恢复时间,并采用台盼蓝拒染法检测两组造血干细胞的活性。结果两组单个核细胞(MNC)和CD34+细胞输入值、台盼蓝拒染率、移植后中性粒细胞(ANC)<0.5×109/L时间、血小板(PLT)<50×109/L时间比较差异无显著性意义(P>0.05)。结论采用3%羟乙基淀粉、4%白蛋白和5%二甲亚砜(DMSO)组成的冷冻保护剂,通过低温冰箱转液氮阶梯降温法冷冻保存造血干细胞,可以达到和传统采用10%DMSO作为冷冻保护剂的程序降温冷冻保存法一样的临床移植效果,缩短了冷冻操作时间,减少了冷冻成本。Objective To explore the influence of cryopreservation by ladder-style freezing from low temperature refrigerator to liquid nitrogen on hemopoietic stem cell（ HSC）transplantation. Methods From April 2001 to April 2006,31 cases treated with HSC transplantation were randomized to controlled-rate freezer-liquid nitrogen group（ n = 15 ）and refrigerator-liquid nitrogen group（ n = 16）in Department of Hematology of 1 st People＇s Hospital of Yunnan Province. The hemopoietic reconstitution time was observed, and the viability of HSC was determined by trypan blue exclusion test in two groups. Results No significant differences were found in transfusion values of mononnclear cells （ MNC ） and CD34^＋ cells,rates of trypan blue exclusion and time of ANC 〈 0. 5 × 10^9/L and PLT 〈 50 × 10^9/L .between two groups （ P 〉 0. 05）. Conclusion Cryopreservation of HSC can produce successful engraftment by ladder-style freezing which has cryoprotectant solution containing final concentrations of 3-percent hydroxyethyl starch ,4-percent human serum albumin, and 5-percent DMSO, and its effect is the same as traditional controlled-rate method with 10-percent DMSO cryoprotectant. The new freezing procedure is faster and easier, and freezing cost is reduced.

关 键 词：造血干细胞移植 冷冻保存 血液病 恶性 

分 类 号：R5[医药卫生—内科学]