玉竹基因组DNA的提取与分析  

Extraction and analysis of DNA in Polygonatum odoratum

作　　者：陈玉秀[1] 周日宝[1] 潘清平[1] 童巧珍[1] 陈言[1] 刘湘丹[1] 

机构地区：[1]湖南中医药大学药学院,湖南长沙410007

出　　处：《西北药学杂志》2007年第4期171-173,共3页Northwest Pharmaceutical Journal

基　　金：国家科技部十五重点攻关项目(编号:2004BA721A27)

摘　　要：目的以玉竹根茎为材料,寻找一种适合于玉竹总DNA提取的方法,便于下游分子生物学的操作。方法采用经典CTAB法与改良CTAB法。结果改良CTAB法提取得到的DNA可以很好地用于PCR扩增。结论改良CTAB法适用于类似玉竹这类含多糖较多的根及根茎类中药材DNA的提取。Objective To establish a extraction method for high-quality DNA from Polygonatum odoratum . Method The samples were extracted by the classic technique of CTAB and improved CTAB. Results DNA extracted with improved CTAB from fresh samples of Polygonaturn odoratum was successfully amplified with PCR technique. Conclusion The improved CTAB technique can be used for extracting DNA from herbs riching polysaccharide.

关 键 词：玉竹 多糖 DNA提取 CTAB法 

分 类 号：R284[医药卫生—中药学]